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Mauricio Claudio Vergara Castro

Researcher

Pontificia Universidad Católica de Valparaíso

Valparaiso, Chile

Cell culture engineering; Protein and metabolite production; Modeling and simulation of metabolic processes, evaluation of bioactive molecules.

Biotechnology , Universidad Tecnica Federico Santa Maria . Chile, 2012
Engineer sciences, PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE. Chile, 2005
Biochemical engineer , PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE. Chile, 2006

Professor part-time Other

PONTIFICIA UNIVERSIDAD CATOLICA DE VALPARAISO

ENGINEERING

Valparaiso, Chile

2008 - 2017

Professor part-time Other

Pontificia Universidad Católica de Valparaíso

Valparaiso, Chile

2008 - 2017
Researcher Part Time

Cellus/PUCV

Valparaiso, Chile

2019 - 2019
Researcher Full Time

Centro Regional en Estudios en Alimentos Saludables (CREAS)

Valparaiso, Chile

2020 - 2021

1. Students: Maria José Sepúlveda . Chemistry and pharmacy- Universidad de Valparaíso. Thesis title: “Evaluation of the antiproliferative activity of lectins obtained from Musa Cavensish on oncogenic cells”. 2018. Director: Mauricio Vergara Castro; Co-director: Jacqueline Concha.
2. Students: Óscar Gatica. Biochemical Engineering- Pontificia Universidad Católica de Valparaíso. Thesis title: “Characterization and selection of a CHO cell line producing innovative monoclonal antibody anti-MICA for cancer treatment”. 2019. Director: Claudia Altamirano Goméz; Co-director: Mauricio Vergara Castro.
3. Students: Karen Segovia. Medical technology- Universidad Santo Tomás . Thesis title: “PROTOCOL DESIGN FOR THE BIOLOGICAL EVALUATION OF BIOACTIVE MOLECULES IN MAMMAL CELLS”. 2020. Director: Alejandra Arancibia; Co-director: Mauricio Vergara Castro.
4. Students: Macarena Fernandez. Biochemical Engineering- Pontificia Universidad Católica de Valparaíso. Thesis title: “Development of a 3D culture model of SH-SY5Y neuroblastoma cells in bioreactor for the biological evaluation of extracts de café”. 2020. Director: Claudia Altamirano Goméz; Co-director: Mauricio Vergara Castro.
5. Students: Óscar Gatica. Master of Engineering Sciences, Biochemical Engineering- Pontificia Universidad Católica de Valparaíso. Thesis title: “Development of a rh-EPO production strategy in c-Myc overexpressing CHO cells in batch culture, based on lactate re-metabolization”. 2020. Director: Claudia Altamirano Goméz; Co-director: Mauricio Vergara Castro.

During the last 10 years I have participated in the dissemination of the research that we carry out in the production of biopharmaceuticals. Firstly, nationally through the CONICYT Explora program, through the participation of the activity 1000 scientists 1000 classrooms, framed in the CONICYT science and technology week. I have made this diffusion for students of basic and secondary education, in private subsidized and municipal schools.
Second, at the international level through the participation of different congresses and seminars in the field of Biotechnology, such as the European Biotechnology congress, and specifically in the area of in vitro culture technology for mammalian cells, such as the "European Society of Animal Cell Technology (ESACT) Meeting "and the" Latin American Symposium on Cell Culture Technology (SLATCC) ".

Scholarship ICGEB to attend theoretical and practical course “Flow Cytometry and Cell Sorting in Biotechnology and Biomedicine Research

INSTITUT PASTEUR

Uruguay, 2014

Assistance and stay to carry out Flow Cytometry course at the Pasteur Institute of Uruguay
MECESUP

UNIVERSIDAD TECNICA FEDERICO SANTA MARIA

Chile, 2011

Beca para realizar estadía doctoral en RICE University de Houston Texas USA por 3 meses.
Thesis support scholarship

CONICYT

Chile, 2010

Scholarship to support the thesis for the purchase of materials
Doctoral scholarship

CONICYT

Chile, 2007

Doctoral scholarship for the completion of a doctorate in Chile.
Carmen Gloria Arevalo Award

EIB PUCV

Chile, 2006

Prize for the best research thesis of the biochemical engineering career of the generation 2006.


Engineering of Chinese hamster ovary cells for co-overexpressing MYC and XBP1s increased cell proliferation and recombinant EPO production Latorre, Yesenia; Torres, Mauro; Vergara, Mauricio; Berrios, Julio; Sampayo, Maria Molina; Goedecke, Natasha; Wirth, Dagmar; Hauser, Hansjoerg; Dickson, Alan J.; Altamirano, Claudia Article ISI SCIENTIFIC REPORTS (2023)
Development of solid-state fermentation process of spent coffee grounds for the differentiated obtaining of chlorogenic, quinic, and caffeic acids Aranciabia-Diaz, Alejandra; Astudillo-Castro, Carolina; Altamirano, Claudia Victoria; Soto-Maldonado, Carmen; Vergara, Mauricio Claudio; Córdova, Andres; Zuñiga, María Elvira Article JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE (2022)
Dynamic Culture of Mesenchymal Stromal/Stem Cell Spheroids and Secretion of Paracrine Factors Fuentes, Paloma; Torres, María José; Arancibia, Rodrigo; Aulestia, Francisco; Vergara, Mauricio Claudio; Carrión, Flavio; Osses, Nelson; Altamirano, Claudia Victoria Article FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY (2022)
Enhanced recombinant protein production in CHO cell continuous cultures under growth-inhibiting conditions is associated with an arrested cell cycle in G1/G0 phase Avello Verónica; Torres, Mauro; Vergara, Mauricio Claudio; Berrios, Julio; Valdez-Cruz N.A.; Acevedo, Cristian; Molina, Maria Carmen; Dickson, Alan J.; Altamirano, Claudia Article PLOS ONE (2022)
In vitro Notch-mediated adjuvant immunogenic potency is induced by combining QS-21 and MPL in a co-culture model of PBMC and HUVEC cells Campos-Estrada, C.; Riquelme, B.; Vergara, M.; Altamirano, C.; Cavieres, M.F. Article Toxicology In Vitro (2020)
Polyphenolic extracts of walnut (Juglans regia) green husk containing juglone inhibit the growth of HL-60 cells and induce apoptosis Soto-Maldonado, Carmen; Vergara-Castro, Mauricio; Jara-Quezada, John; Caballero-Valdés, Eduardo; Müller-Pavez, Andrea; Zúñiga-Hansen, María and Altamirano Article ELECTRONIC JOURNAL OF BIOTECHNOLOGY (2019)
Metabolic flux analysis during galactose and lactate co-consumption reveals enhanced energy metabolism in continuous CHO cell cultures Torres, Mauro; Berrios, Julio; Rigual, Yandi; Latorre, Yesenia; Vergara, Mauricio; Dickson, Alan J.; Altamirano, Claudia Article ISI SCOPUS CHEMICAL ENGINEERING SCIENCE (2019)
Polyphenolic extracts of walnut (Juglans regia) green husk containing juglone inhibit the growth of HL-60 cells and induce apoptosis Soto-Maldonado, Carmen; Vergara, Mauricio; Jara, John; Caballero, Valdez, Eduardo; Müller, Andrea; Zuñiga-Hansen, Maria E; Altamirano, Claudia Article ELECTRONIC JOURNAL OF BIOTECHNOLOGY (2019)
High glucose and low specific cell growth but not mild hypothermia improve specific r-protein productivity in chemostat culture of CHO cells Vergara, Mauricio; Torres, Mauro; Müller, Andrea; Avello, Varonica; Acevedo, Cristian; Berrios, Julio; Reyes, Juan G.; Valdes-Cruz, Norma A; Altamirano, Claudia Article PLOS ONE (2018)
Mild hypothermia improves anti-TNFα production and upregulates expression of myc and xbp1s in CHO cells Torres, Mauro; Zuñiga, Roberto; Gutierrez, Matias; Vergara, Mauricio; Collazo, Norberto; Reyes, Juan G.; Berrios, Julio; Aguillon, Juan C; Molina, María Carmen; Altamirano, Claudia Article PLOS ONE (2018)
Impact of sodium butyrate and mild hypothermia on metabolic and physiological behavior of CHO TF 70R cells Avello, Veronica; Tapia, Bethzabeth; Vergara, Mauricio; Acevedo, Cristian; Berrios, Julio; Reyes, Juan G.; Altamirano, Claudia Article ELECTRONIC JOURNAL OF BIOTECHNOLOGY (2017)
Application of a new model based on oxygen balance to determine the oxygen uptake rate in mammalian cell chemostat cultures Rigual-González, Y Article ISI SCOPUS CHEMICAL ENGINEERING SCIENCE (2016)
Antiproliferative evaluation of tall-oil docosanol and tetracosanol over CHO-K1 and human melanoma cells Vergara M.; Olivares, A.; Altamirano, C Article ISI SCIELO SCOPUS ELECTRONIC JOURNAL OF BIOTECHNOLOGY (2015)
Endoplasmic Reticulum-Associated rht-PA Processing in CHO Cells: Influence of Mild Hypothermia and Specific Growth Rates in Batch and Chemostat Cultures Vergara M.; Berrios J.; Martínez I.; Diaz-Barrera, A; Acevedo, C; Reyes JG.; González R.; Altamirano, C Article ISI SCOPUS PLOS ONE (2015)
Characterization of pressurized hot water extracts of grape pomace: Chemical and biological antioxidant activity Vergara-Salinas J.R.; Vergara M.; Altamirano, C; González A; Perez Correa J.R. Article SCOPUS FOOD CHEMISTRY (2014)
Differential Effect of Culture Temperature and Specific Growth Rate on CHO Cell Behavior in Chemostat Culture Vergara, Mauricio; Becerra, Silvana; Berrios, Julio; Osses, Nelson; Reyes, Juan; Rodríguez-Moyá, María; Gonzalez, Ramon; Altamirano, Claudia; Dumont, Jacques Emile Article ISI SCOPUS PLOS ONE (2014)
Simultaneous environmental manipulations in semi-perfusion cultures of CHO cells producing rh-tPA Vergara M.; Becerra, S; Diaz-Barrera, A; Berrios J.; Altamirano, C Article ISI SCOPUS ELECTRONIC JOURNAL OF BIOTECHNOLOGY (2012)


Condition of mild hypothermia does not promote an increase in specific productivity of recombinant protein at high specific growth rate Becerra, S; Vergara M.; González R.; Osses N.; Altamirano, C Abstract ISI CURRENT OPINION IN BIOTECHNOLOGY (2011)
Condition of Mild Hypothermia does not Promote an Increase in Specific Productivity of Recombinant Protein in Continuous Culture of CHO Cells Becerra, S; Vergara M.; Gozalez, R; Osses N.; Altamirano, C Abstract ISI JOURNAL OF BIOTECHNOLOGY (2010)
Incidence of Mild Hypothermia on Metabolic Behavior and Synthesis of tPA in CHO Cells Inhibited ERAD Degradation Pathways Vergara M.; Becerra, S; Reyes, J; Altamirano, C Abstract ISI JOURNAL OF BIOTECHNOLOGY (2010)
Galactose and lactate as nutrients in continuous cultures of CHO cells producing rh-tPA Altamirano, C; Berrios J.; Vergara M. Abstract ISI NEW BIOTECHNOLOGY (2009)
Glycosylation stage leads production of extracellular rh-tPA at sub-physiological temperatures of culture Vergara M.; Becerra, S; Altamirano, C Abstract ISI NEW BIOTECHNOLOGY (2009)
Metabolic behavior of CHO cells cultured in perfusion with handling simultaneous of low temperature and source of carbon Vergara M.; Becerra, S; Berrios J.; Altamirano, C Abstract ISI NEW BIOTECHNOLOGY (2009)
Simultaneus effect of low temperatures and mannose concentration on CHP cells cultures producing rh-tPA Altamirano, C; Berrios J.; Diaz-Barrera, A; Bazan C.; Vergara M. Abstract ISI NEW BIOTECHNOLOGY (2009)
Effect of low temperatures on metabolism of CHO cells cultured on different carbon and energy sources Berrios J.; Paredes C.; Vergara M.; Altamirano, C Abstract ISI JOURNAL OF BIOTECHNOLOGY (2007)


Basic Aspect of Animal Cell Cultivation Altamirano, Claudia; Berrios, Julio; Vergara, Mauricio; Hauser, Hansjörg; Wagner, Roland BookSection (2015)


The overexpression of transcripción factor, Xbp1s alone and jointly with oncogene c-myc, has a variable impact on the productivity of rh-erythropoietin expressed in CHO cells Daniela Araya; Mauricio Vergara; Yesenia Latorre; Claudia, Altamirano ConferencePoster (2019)
Alta concentración de glucosa en cultivo en quimiostato de células CHO mejora la productividad específica de la proteína rht-PA Vergara, M.; Müler, A.; Avello, V.; Acevedo, C.; Berríos, J.; Reyes, J.G.; Altamirano, C. ConferencePoster (2017)
Aplicación de un nuevo modelo basado en el balance de oxígeno para determinar la VCO en cultivos continuos de células de mamífero Yandi Rigual; Lucía Gómez; Jonathan Núñez; Mauricio Vergara; Álvaro Díaz; Julio Berríos; Claudia, Altamirano ConferencePoster (2017)
High Glucose Concentration and Low Specific Cell Growth Rate Improve Specific r-tPA Productivity in Chemostat Culture of CHO Cells Vergara, Mauricio; Müller, Andrea; Avello, Veronica; Acevedo, Cristian; Berrios, Julio; Reyes, Juan G.; Altamirano, Claudia ConferencePoster (2017)
Nuevo modelo para determinar la VCO en cultivos continuos de células de mamífero para la producción de biofármacos Yandi Rigual; Lucía Gómez; Jonathan Núñez; Mauricio Vergara; Álvaro Díaz; Julio Berríos; Claudia, Altamirano ConferencePoster (2017)
Desarrollo de una estrategia de producción de anti-TNF en células CHO bajo condiciones de hipotermia suave Roberto Zúñiga; Mauricio Vergara; Norberto Collazo; Mauro Torres; Matías Gutierrez; Juan Reyes; Julio Berrios; Juan Carlos Aguillón; María Carmen Molina; Claudia, Altamirano ConferencePoster (2016)
Hipotermia suave como estrategia para maximizar la producción de anti-tnf en cultivo batch de celulas CHO Roberto Zúñiga; Mauricio Vergara; Norberto Collazo; Mauro Torres; Matías Gutierrez; Juan Reyes; Julio Berríos; Juan Carlos Aguillón; María Carmen Molina; Claudia, Altamirano ConferencePoster (2016)
Identification of molecular networks involved in specific productivity increase of t-PA produced in chemostat culture of CHO cells under conditions of mild hypothermia, addition of sodium butyrate and variation of the specific cell growth rate Vergara, Mauricio; Avello, Verónica; Acevedo, Cristian; Berrios, Julio; Reyes, Juan G.; Altamirano, Claudia ConferencePoster (2016)
Perfil transcriptómico de la línea celular CHO TF 70R expuesta a hipotermia leve y butirato de sodio a velocidad de dilución definida Verónica Avello; Mauricio Vergara; Cristian Acevedo; Claudia, Altamirano ConferencePoster (2016)
Evaluation of the differential effect of specific growth rate and cultivation temperature on production of recombinant proteins by chemostat cultures of CHO cells Vergara, Mauricio; Muñoz, María J.; Berrios, Julio; Reyes, Juan G.; Altamirano, Claudia ConferencePoster (2015)
Galactose and lactate co-metabolism in continues culture of CHO cell M.T. Vargas; Y. Latorre; J. Berríos; C. Altamirano; M. Vergara ConferencePoster (2015)
Mild hypothermia applied to production of recombinant proteins in CHO cell cultures C. Altamirano; M. Vergara; J. Berríos ConferencePoster (2015)
Relation between tissue plasminogen activator (tPA) productivity and cell cycle phases of CHO cells growth in chemostat culture Avello, Verónica; Tapia, Bethzabeth; Vergara, Mauricio; Acevedo, Cristian; Altamirano, Claudia ConferencePoster (2015)
Efecto sobre la productividad de t-PA en células CHO expuestas a hipotermia suave y butirato de sodio Tapia, B.; Avello, V.; Vergara, M.; Acevedo, C.; Altamirano, C. ConferencePoster (2014)
Effect of sodium butyrate on specific productivity of CHO-tPA cells adapted to mild hypothermia Avello, Verónica; Tapia, Bethzabeth; Vergara, Mauricio; Acevedo, Cristian; Altamirano, Claudia ConferencePoster (2014)
Induction of apoptosis and loss of membrane potential in CHO cells exposed to mild hypothermia and sodium butyrate Verónica Avello; Betzabeth Tapia; Mauricio Vergara; Claudia, Altamirano ConferencePoster (2014)
Parámetros metabólicos involucrados en la producción de rh-GM-CSF en cultivos continuos de células CHO sometidos a hipotermia suave Muñoz M.J.; Reyes, J.; Berrios, J.; Vergara, M.; Altamirano, C. ConferencePoster (2014)
Protein folding and glycosylation process are influenced by subphysiological temperatures in batch culture and by specific growth rate in continuous cultures of CHO cells producing rt-PA Vergara, Mauricio; Becerra, Silvana; Berrios, Julio; Acevedo, Cristian; Reyes, Juan G.; Gonzalez, Ramon; Osses, Nelson; Altamirano, Claudia ConferencePoster (2013)
Application of inhibitors of the translation process and ERAD pathways leading to increased specific productivity of t-PA in CHO cells in continuous culture Vergara, Mauricio; Becerra, Silvana; Berrios, Julio; Reyes, Juan G.; Gonzalez, Ramon; Altamirano, Claudia ConferencePoster (2012)
Efecto de temperatura de cultivo y velocidad especifica de crecimiento sobre productividad específica de t-PA producido por células CHO en cultivo continuo Mauricio Vergara; Silvana Becerra; Julio Berrios; Cristian Acevedo; Nelson Osses; Juan Reyes; Ramón González; Claudia, Altamirano ConferencePoster (2012)
Estudio del amonio endógeno y actividad glicolitica en células cho-tpa cultivadas a temperatura subfisiológicas Cristian Acevedo; Julio Berrios; Mauricio Vergara; Isolee Espeiñeira; Paula Cifuentes; Claudia, Altamirano ConferencePoster (2012)
Subphysiological temperatures influence glycosylation process in CHO cells producing recombinant human t-PA Vergara, Mauricio; Becerra, Silvana; Berrios, Julio; Acevedo, Cristian; Reyes, Juan G.; Altamirano, Claudia ConferencePoster (2012)
Antioxidant properties of extract antioxidant deriving out of solid residues from chilean native mix of berries: in vitro culture test Muñoz, María J.; Vergara, Mauricio; Becerra, Silvana; Zuñiga, María E.; Altamirano, Claudia ConferencePoster (2011)
Condición de hipotermia suave no promueve aumento en productividad especifica de proteína recombinante en cultivo continuo de células CHO Silvana Becerra; Mauricio Vergara; Ramón González; Nelson Osses; Claudia, Altamirano ConferencePoster (2010)
Etapas post-traduccionales punto clave en la producción de proteína recombinante tPA a bajas temperaturas Mauricio Vergara; María José Muñoz; Julio Berríos; Juan Reyes; Claudia, Altamirano ConferencePoster (2010)
Incidence of mild hypothermia on metabolic behavior and synthesis of tPA in cells inhibited ERAD degradation pathways Vergara, Mauricio; Becerra, Silvana; Reyes, Juan G.; Altamirano, Claudia ConferencePoster (2010)
Comparación de dos modelos de crecimiento bajo doble limitación de nutrientes en cultivo continuo Fernando Acevedo; Lorena Alvarez; Romina Alvarez; Mauricio Vergara ConferencePoster (2009)
Eventos fisiológicos tempranos inducidos por transiciones de temperatura en células CHO Tamara Marín; Silvana Becerra; Mauricio Vergara; Claudia, Altamirano; Nelson Osses; Juan G Reyes ConferencePoster (2009)
Simultaneus effect of low temperatures and mannose concentration on CHO cells cultures producing rh-tPA C. Altamirano; J. Berrios; A. Díaz-Barrera; C. Bazan; M. Vergara ConferencePoster (2009)
Use of system biology approach to identification of key stage in the production of recombinant glycoprotein therapeutics in mammalian cell culture Silvana Becerra; Mauricio Vergara; Nelson Osses; Claudia, Altamirano ConferencePoster (2009)
Effect of different carbon sources and low temperaturas on metabolic behavior of mammalian cells Silvana Becerra; Julio Berrios; Mauricio Vergara; Nelson Osses; Claudia, Altamirano ConferencePoster (2008)
Effect of enviromental simultaneus manipulations in the development of perfusion cultures of CHO cells Mauricio Vergara; Silvana Becerra; Claudia, Altamirano ConferencePoster (2008)
Use of substrates slowly metabolised and subphysiological temperaturas in CHO cell cultures Silvana Becerra; Mauricio Vergara; Claudia, Altamirano ConferencePoster (2008)
Efecto de bajas temperaturas sobre el metabolismo de células CHO cultivadas en diferentes fuentes de carbono y energía Iván Paredes; Mauricio Vergara; Claudia, Altamirano ConferencePoster (2006)


Aplicación de una tecnología enzimática para la recuperación de biomoléculas de alto potencial comercial a partir del aprovechamiento integral del bagazo cervecero Soto-Maldonado, Carmen; Berrios, Julio; Olivares, Araceli; Vergara, Mauricio Claudio Proyecto (2022)
Design and production of completely human recombinant monoclonal antibodies through a multidisciplinary approach based on the development of optimized strategies Altamirano, Claudia Victoria; Berrios, Julio; Molina, Maria Carmen; KOGAN, MARCELO; Hermoso, Marcela; Toledo, Jorge; Vergara, Mauricio Claudio Proyecto (2021)
Desarrollo de un nuevo biofármaco con actividad anti-tumoral para el tratamiento de cáncer: Anticuerpo monoclonal completamente humano dirigido contra el sitio de combinación de MICA a su receptor de activación Maria Carmen Molina; Mauricio Vergara Castro; Claudia, Altamirano Proyecto (2020)
Development of a Strategy for the Production of Recombinant Human Erythropoietin in CHO over-expressing c-myc Cells Based on Lactate Consumption in High Cell Density Culture Mauricio Vergara Castro Proyecto (2019)
Desarrollo de prototipo acelular para regeneración tisular en base a secretoma de MSCs en 3D cultivadas en biorreactor, con potencial de exportación Grupo Cellus Proyecto (2018)
Efecto diferencial de la velocidad específica de crecimiento sobre comportamiento productivo y metabólico de células CHO en cultivo en quimiostato bajo condiciones de hipotermia suave y crecientes concentraciones de glucosa FONDECYT (3150373) Proyecto 2015-2017 Inv. Responsable
Efecto diferencial de la velocidad específica de crecimiento sobre el comportamiento metabólico y productivo de células CHO en cultivo en quimiostato bajo condiciones de hipotermia suave y crecientes contrentraciones de glucosa Mauricio Vergara Castro Proyecto (2015)
Evaluation of the differential effect of specific growth rate and cultivation temperature on production of recombinant proteins by continuous cultures of CHO cells FONDECYT (1120667) Proyecto 2012-2015 Investigador(a)
Effect of concentration of different carbon sources and low temperatures on metabolic flux distribution, specific productivity and quality of tPA in chinese hamster ovary cells FONDECYT (1070337) Proyecto 2007-2009 Investigador(a)
DESARROLLO DE BIOCONTROLADORES DE LISTERIA MONOCYTOGENES PARA SU INCORPORACION AL PROCESAMIENTO INDUSTRIAL DEL SALMON FONDEF (D04I1153) Proyecto 2004-2008 Personal técnico y/o de apoyo
RECUPERACION Y PURIFICACION DE ALCOHOLES ALIFATICOS DE CADENA LARGA DE LOS RESIDUOS DEL PROCESO KRAFT DE PULPEO DE LA CELULOSA PARA SU USO EN LA INDUSTRIA FARMACEUTICA FONDEF (D04I1007) Proyecto 2004-2007 Personal técnico y/o de apoyo


Advances in improving mammalian cells metabolism for recombinant protein production Altamirano, Claudia; Berrios, Julio; Vergara, Mauricio; Becerra, Silvana Review ISI SCOPUS ELECTRONIC JOURNAL OF BIOTECHNOLOGY (2013)