Assessment of the IgG index in dogs by indirect immunoenzimatic assays as diagnostic tool for inflammatory diseases of central nervous system

Mejias, M; Molina, MC; Ferreira A.

Abstract

The IgG index measures the intrathecal immunoglobulin production and it is a useful tool for diagnosis of inflammatory diseases involving the central nervous system. This index is based on the precise quantification of albumin and IgG in canine cerebrospinal fluid and serum. Here, we report the development of an indirect competitive ELISAs for the detection of both antigens. Thirty-two dogs were included in this study, divided into three experimental groups. Group A was composed of 22 healthy animals, as determined by standard clinical examination. In group B, six animals, presented neurological pathologies associated with endogenous IgG production and, in group C four animals presented neurological diseases or symptoms not associated with intrathecal IgG production. Cerebrospinal fluid and serum samples were obtained from these animals. As expected, by using the indirect ELISAs proposed here, the IgG indexes obtained in healthy animals (A) were 0.371 ± 0.252 (SD). In B and C, the values (3.002 ± 1.897; 0.36 ± 0.306, respectively), were in agreement with the pathologic conditions of the individuals in each group. Thus, the immunometric competition ELISA methods proposed here allow the discrimination of abnormal intrathecal IgG production, in a variety of inflammatory pathologic conditions of the central nervous system. © 2007.

Más información

Título según WOS: Assessment of the IgG index in dogs by indirect immunoenzimatic assays as diagnostic tool for inflammatory diseases of central nervous system
Título según SCOPUS: Assessment of the IgG index in dogs by indirect immunoenzimatic assays as diagnostic tool for inflammatory diseases of central nervous system
Título de la Revista: RESEARCH IN VETERINARY SCIENCE
Volumen: 84
Número: 3
Editorial: ELSEVIER SCI LTD
Fecha de publicación: 2008
Página de inicio: 335
Página final: 340
Idioma: English
URL: http://linkinghub.elsevier.com/retrieve/pii/S0034528807001774
DOI:

10.1016/j.rvsc.2007.06.011

Notas: ISI, SCOPUS