Differential distribution of the Sodium-vitamin C cotransporter-1 along the proximal tubule of the mouse and human kidney

Castro, T.; Low M; Salazar, K.; Montecinos, H.; Cifuentes M.; Yanez, AJ; Slebe, JC; Figueroa, CD; Reinicke K; Garcia, MD; Henriquez, JP; Nualart, F.

Abstract

Vitamin C is reabsorbed from the renal lumen by one isoform of sodium-vitamin C co-transporters that mediate high affinity sodium-dependent L-ascorbic acid transport. Sodium-vitamin C cotransporter-1 mRNA has been detected in intestine and liver and the S3 segment of the renal proximal tubule. Here, we found that its distribution was broader and all three proximal tubule segments of mouse and human expressed the transporter but the S3 segment had the highest expression. Sodium-vitamin C co-transporter-1 expression was also found in the renal epithelial-derived LLC-PK1 cell line. Ascorbic acid transport in these cells was regulated by a single kinetic component that depended on the sodium concentration, pH and temperature. Reducing ascorbate concentration increased the apical expression of the transporter suggesting the presence of a feedback system for regulation of transporter abundance at the luminal membrane. © 2008 International Society of Nephrology.

Más información

Título según WOS: Differential distribution of the Sodium-vitamin C cotransporter-1 along the proximal tubule of the mouse and human kidney
Título según SCOPUS: Differential distribution of the sodium-vitamin C cotransporter-1 along the proximal tubule of the mouse and human kidney
Título de la Revista: KIDNEY INTERNATIONAL
Volumen: 74
Número: 10
Editorial: Elsevier Science Inc.
Fecha de publicación: 2008
Página de inicio: 1278
Página final: 1286
Idioma: English
URL: http://www.nature.com/doifinder/10.1038/ki.2008.329
DOI:

10.1038/ki.2008.329

Notas: ISI, SCOPUS