Plasmid-mediated bacteriocin production by Shigella flexneri isolated from dysenteric diarrhoea and their transformation into Escherichia coli

Padilla, C; Lobos O.; Brevis, P; Abaca, P; Hubert E.

Abstract

Aims: To determine the production of bacteriocin by Shigella flexneri strains, to relate their production to the presence of dysenteric diarrhoea and to asses the genetic determination of the bacteriocin. Methods and Results: One hundred and sixteen strains of Sh. flexneri were isolated from patients with diarrhoea and 49 of them produced bacteriocin active against several Escherichia coli and abacteriocinogenic Sh. flexneri strains. The extrachromosomal DNA isolated from bacteriocinogenic Sh. flexneri strains were used as a substrate to transform E. coli HB-101 cells by means of electroporation. Conclusions: Only the Sh. flexneri strains isolated from dysenteric diarrhoea produced bacteriocin. It was demonstrated that a plasmid of approx. 3 kb was responsible for the genetic determination of these anti-bacterial substances. Significance and Impact of the Study: A 3-kb plasmid that harboured information for the production of bacteriocin by Sh. flexneri strains was described. The production of this bacteriocin may be related to dysenteric diarrhoea produced by these bacterial strains. © 2006 The Society for Applied Microbiology.

Más información

Título según WOS: Plasmid-mediated bacteriocin production by Shigella flexneri isolated from dysenteric diarrhoea and their transformation into Escherichia coli
Título según SCOPUS: Plasmid-mediated bacteriocin production by Shigella flexneri isolated from dysenteric diarrhoea and their transformation into Escherichia coli
Título de la Revista: LETTERS IN APPLIED MICROBIOLOGY
Volumen: 42
Número: 3
Editorial: Wiley
Fecha de publicación: 2006
Página de inicio: 300
Página final: 303
Idioma: English
URL: http://doi.wiley.com/10.1111/j.1472-765X.2005.01829.x
DOI:

10.1111/j.1472-765X.2005.01829.x

Notas: ISI, SCOPUS