ATPase and ADPase activities in synovial membrane of equine metacarpophalangeal joint

Jimenez, PA; Garcia, L.; Adarmes, H; Gonzalez E.; Valenzuela, MA.

Abstract

ATPase and ADPase activities capable of hydrolyzing nucleoside di- and triphosphates in the presence of Ca2+ are present in synovial membrane of metacarpophalangeal joint mainly associated to membrane fractions. These hydrolytic activities have been considered involved in the inflammatory process where ATP and ADP are inflammatory mediators while adenosine counteracts this effect. Both, subcellular localization and kinetic properties of these nucleotidase activities, suggest that could correspond to single enzyme called ATP-diphosphohydrolase or apyrase. The comparison of the activity on ATP-Ca and ADP-Ca from normal and pathological equine synovial membrane did not show significant differences either in the subcellular fraction distribution or in the enrichment of each subcellular fraction. Neither differences on 5?-nucleotidase activity present in the microsomal fraction were observed. © 2002 Elsevier Science Inc. All rights reserved.

Más información

Título según WOS: ATPase and ADPase activities in synovial membrane of equine metacarpophalangeal joint
Título según SCOPUS: ATPase and ADPase activities in synovial membrane of equine metacarpophalangeal joint
Título de la Revista: LIFE SCIENCES
Volumen: 70
Número: 20
Editorial: PERGAMON-ELSEVIER SCIENCE LTD
Fecha de publicación: 2002
Página de inicio: 2445
Página final: 2455
Idioma: English
URL: http://linkinghub.elsevier.com/retrieve/pii/S0024320502015291
DOI:

10.1016/S0024-3205(02)01529-1

Notas: ISI, SCOPUS