Synthesis and In Vivo Evaluation of the Biodistribution of a F-18-Labeled Conjugate Gold-Nanoparticle-Peptide with Potential Biomedical Application

Guerrero, S; Herance J.R.; Rojas, S.; Mena J.F.; Gispert J.D.; Acosta G.A.; Albericio, F.; Kogan, M. J.

Abstract

Gold nanoparticles (AuNPs) have been extensively used in biological applications because of their biocompatibility, size, and ease of characterization, as well as an extensive knowledge of their surface chemistry. These features make AuNPs readily exploitable for biomedical applications, including drug delivery and novel diagnostic and therapeutic approaches. In a previous work, we studied ex vivo distribution of the conjugate C(AuNP)-LPFFD for its potential uses in the treatment of Alzheimer's disease. In this study, we covalently labeled the conjugate with [ 18F]-fluorobenzoate to study the in vivo distribution of the AuNP by positron emission tomography (PET). After intravenous administration in rat, the highest concentration of the radiolabeled conjugate was found in the bladder and urine with a lower proportion in the intestine, demonstrating progressive accumulation compatible with biliary excretion of the conjugate. The conjugate also accumulated in the liver and spleen. PET imaging allowed us to study the in vivo biodistribution of the AuNPs in a noninvasive and sensitive way using a reduced number of animals. Our results show that AuNPs can be covalently and radioactively labeled for PET biodistribution studies. © 2012 American Chemical Society.

Más información

Título según WOS: Synthesis and In Vivo Evaluation of the Biodistribution of a F-18-Labeled Conjugate Gold-Nanoparticle-Peptide with Potential Biomedical Application
Título según SCOPUS: Synthesis and in vivo evaluation of the biodistribution of a 18F-labeled conjugate gold-nanoparticle-peptide with potential biomedical application
Título de la Revista: BIOCONJUGATE CHEMISTRY
Volumen: 23
Número: 3
Editorial: AMER CHEMICAL SOC
Fecha de publicación: 2012
Página de inicio: 399
Página final: 408
Idioma: English
URL: http://www.scopus.com/inward/record.url?eid=2-s2.0-84858742858&partnerID=40&md5=6c86485e37cf7946259734aa7f574db9
DOI:

10.1021/bc200362a

Notas: ISI, SCOPUS