Over-expression of forkhead box P3 and its association with receptor activator of nuclear factor-? B ligand, interleukin (IL) -17, IL-10 and transforming growth factor-? during the progression of chronic periodontitis

Vernal, R; Dutzan N.; Gamonal, J; Silva, A; Sanz, M.

Keywords: acid, proteins, growth, differentiation, dna, hormone, expression, cytokines, interferon-gamma, cytokine, transcription, binding, cells, protein, cell, gene, interleukin-10, disease, chain, periodontitis, ligand, down-regulation, humans, human, genetics, receptor, polymerase, regulation, domain, nuclear, gamma, progression, thyroid, bone, dna-binding, article, lymphotoxin-alpha, factor, immunology, interferon, lymphocyte, chronic, th1, foxp3, box, reverse, rank, factors, helper-inducer, study, 3, loss, interleukin, interleukin-17, Reaction, Receptors,, comparative, t, beta1, course, 1beta, regulatory, protein,, Transcriptase, osteolysis, transforming, retinoic, down, helper, 10, Alveolar, T-Lymphocytes,, T-Box, 17, osteoclast, Forkhead, lymphotoxin, Th2, GATA, orphan, Interleukin-1beta, GATA3, TBX21, ROR, ROR-gamma

Abstract

Dutzan N, Gamonal J, Silva A, Sanz M, Vernal R. Over-expression of forkhead box P3 and its association with receptor activator of nuclear factor-? B ligand, interleukin (IL)-17, IL-10 and transforming growth factor-? during the progression of chronic periodontitis. J Clin Periodontol 2009; 36: 396-403. doi: 10.1111/j.1600-051X.2009.01390.x. Abstract Aim: T regulatory (Treg) cells have been detected in periodontitis lesions, and forkhead box P3 (Foxp3) expression has been negatively correlated to receptor activator of nuclear factor-? B ligand (RANKL). The aim of this study was to correlate T-helper type 1 (Th1), Th2, Th17 and Treg transcription factor expressions, in gingival tissues from patients undergoing active periodontal tissue destruction, with bone loss-associated cytokines. Materials and Methods: In 10 chronic periodontitis patients undergoing disease progression, the mRNA expressions of T-bet, GATA-3, Foxp3, RORC2, interleukin (IL)-1?, IL-10, IL-17, RANKL, interferon (IFN)-? and transforming growth factor (TGF)-?1 were quantified using real-time reverse transcription-polymerase chain reaction. The levels of these markers were compared between active and inactive periodontal lesions. Results: In active periodontal lesions, Foxp3, T-bet, RANKL, IL-17, IL-1? and IFN-? were significantly over-expressed compared with inactive lesions. The expression of IFN-? was the highest within the active periodontal lesions, similar to that of TGF-?1 within the inactive ones. There was a positive correlation between RANKL and IL-17. Additionally, RANKL and IL-17 were positively correlated with RORC2, but no correlation was detected with Foxp3. Conclusions: These results lead us to speculate that Foxp3+ cells that do not have a regulatory function might have a role in the pathogenesis of active periodontal lesions by down-regulating TGF-?1 and IL-10 synthesis that lead to the over-expression of Th17-associated cytokines RANKL and IL-17. © 2009 John Wiley & Sons AS.

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Título de la Revista: JOURNAL OF CLINICAL PERIODONTOLOGY
Volumen: 36
Número: 5
Editorial: Wiley
Fecha de publicación: 2009
Página de inicio: 396
Página final: 403
URL: http://www.scopus.com/inward/record.url?eid=2-s2.0-64949151593&partnerID=q2rCbXpz