Identification of sex chromosome molecular markers using RAPDs and fluorescent in situ hybridization in rainbow trout

Iturra, P; Lam, N; Vergara N.; Marín J.C.; Medrano J.F.; Bagley, M

Keywords: localization, dna, fluorescence, identification, hybridization, trout, sex, molecular, chromosome, marker, rainbow, determination, polymorphic, in, Y, Situ, Random, chromosomal, amplified

Abstract

The goal of this work is to identify molecular markers associated with the sex chromosomes in rainbow trout to study the mode of sex determination mechanisms in this species. Using the RAPD assay and bulked segregant analysis, two markers were identified that generated polymorphic bands amplifying preferentially in males of the Mount Lassen and Scottish strains of rainbow trout. Chromosomal localization using fluorescent in situ hybridization of a 900 bp probe developed from one of these markers revealed a brightly defined signal on a chromosome that could morphologically be classified as the Y chromosome.

Más información

Título de la Revista: GENETICA
Volumen: 101
Número: 3
Editorial: KLUWER ACADEMIC PUBL
Fecha de publicación: 1997
Página de inicio: 209
Página final: 213
URL: http://www.scopus.com/inward/record.url?eid=2-s2.0-0031400634&partnerID=q2rCbXpz