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Lina Maria Ruiz Hincapie

Academico Docente Investigador

Universidad Autónoma de Chile

Santiago, Chile

Líneas de Investigación


Effect of antioxidants on mitochondrial physiology Remodeling of respiratory supercomplexes and energetic metabolism. Regulation of bioenergetics in response to metabolic changes and hypoxia.

Educación

  •  Microbiología, UNIVERSIDAD DE CHILE. Chile, 2010
  •  Magister Biología, Universidad de Antioquia. Colombia, 2003
  •  Biológa, Universidad de Antioquia. Colombia, 1999

Experiencia Académica

  •   Académico Docente Investigador Full Time

    Universidad Autónoma de Chile

    Facultad de Ciencias de la Salud

    Santiago, Chile

    Enero 2013 - A la fecha

  •   Postdoctorante Full Time

    Universidad Andrés Bello

    Facultad de Ciencias Biológicas

    Santiago, Chile

    Enero 2010 - Enero 2013

Experiencia Profesional

  •   Asistente de Investigación Full Time

    Corporación par Investigaciones Biológicas (CIB)

    Medellín, Colombia

    Enero 2000 - Enero 2004

  •   Académico Docente Investigador Full Time

    Universidad Autónoma de Chile

    Santiago, Chile

    Enero 2013 - A la fecha

Formación de Capital Humano


Manuel Carrasco, 2018, Estudio de la Fisiología Mitocondrial en Niños Chilenos con Trastorno del Espectro Autista, Magíster en Neurociencias, Universidad Autónoma de Chile.
Celia Salazar, 2015, Estudio del efecto de la hipoxia y/o cambios en el metabolismo energético sobre la regulación de la expresión del gen higd2a, Biotecnologo. Grado Académico Licenciado en Biotecnología. Universidad Santo Tomás.


Premios y Distinciones

  •   First-place award for best summary oral presentation option INSAR Regional Meeting 2019

    UNIVERSIDAD AUTONOMA DE CHILE

    Chile, 2019

    High Mitochondrial DNA Levels in Autism Spectrum Disorder is Suggestive of Alterations in the Mitochondrial DNA Replisome: Analysis of The Mitochondrial Transcription Factor A INTRODUCTION: Autism Spectrum Disorder (ASD) is a complex neurodevelopmental disorder with a strong genetic basis. Despite this, only a small fraction of the genes that can be the cause of it are known and supported by strong genetic evidence. Recently, our group started the study of the influence of mitochondrial physiology on the development of ASD. In children with ASD, we found a significant increase in mitochondrial DNA (mtDNA) levels. Likewise, in these children, an increase in the protein oxidation and high expression of the MFN2 gene was observed. The increase in mtDNA levels is suggestive of alterations in the mitochondrial DNA replisome where different proteins are involved than those used for the replication of nuclear DNA, such as the mitochondrial Transcription Factor A (TFAM), the TWINKLE helicase, and the DNA polymerase ?, among others. TFAM is an activator of mitochondrial transcription, it also functions as a histone-like in the packaging of mtDNA, and as mtDNA levels follow TFAM levels. OBJECTIVE: Elucidating the role of TFAM in ASD. METHODS: In the present study was used the computational tool from the Lewis–Sigler Institute for Integrative Genomics at the University of Princeton for the prediction of TFAM gene association with the genetic bases of ASD. Moreover, we analyze the datasets of TFAM expression in ASD by profiling arrays with the Gene Expression Omnibus (GEO) repository. Datasets GSE49105 and GSE111176 were analyzed with GEO2R. Finally, we performed a review of TFAM and ASD in literature. RESULTS: The brain-specific functional relationships involving the TFAM gene show the integration with genes that are more likely to be associated with the development of autism is evident. Also, the enrichment of genes shows the involvement in biological processes such as nuclear transport, nucleocytoplasmic transport, and RNA export from the nucleus. Moreover, the enrichment of genes shows some spatiotemporal signatures such as the orbital prefrontal cortex, striatum, and ventrolateral prefrontal cortex in early-mid fetal development; and inferior temporal cortex, amygdala, hippocampus and ventrolateral prefrontal cortex in early fetal development. The analyses of GSE49105 and GSE111176 datasets show that TFAM gene expression in blood is not altered in ASD. In literature, we found the report that there is no significant difference in the levels of TFAM between ASD and control postmortem brain samples. However, the expression of TFAM in the ASD Lymphoblastoid cell lines was reduced at both RNA and protein levels. CONCLUSION: High mtDNA levels in children with ASD may occur at the TFAM transcriptional level in some specific regions of the brain during fetal development. Further analyses are required to understand the role of TFAM in ASD. Our group continues studying the potential alterations in the mitochondrial DNA replisome in ASD.


 

Article (27)

Alterations of Mitochondrial Biology in the Oral Mucosa of Chilean Children with Autism Spectrum Disorder (ASD)
Copper deficiency- induced anemia is caused by a mitochondrial metabolic reprograming in erythropoietic cells
Interpreting Aromaticity and Antiaromaticity through Bifurcation Analysis of the Induced Magnetic Field
The OXPHOS supercomplex assembly factor HIG2A responds to changes in energetic metabolism and cell cycle
Which NICS method is most consistent with ring current analysis? Assessment in simple monocycles
A Fukui function-guided genetic algorithm. Assessment on structural prediction of Si-n (n=12-20) clusters
Proposal of a Simple and Effective Local Reactivity Descriptor through a Topological Analysis of an Orbital-Weighted Fukui Function
Theoretical design of stable hydride clusters: isoelectronic transformation in the EnAl4-nH7+n- series (vol 7, pg 16069, 2017)
Non-cytotoxic copper overload boosts mitochondrial energy metabolism to modulate cell proliferation and differentiation in the human erythroleukemic cell line K562
Diguanylate Cyclase Null Mutant Reveals That C-Di-GMP Pathway Regulates the Motility and Adherence of the Extremophile Bacterium Acidithiobacillus caldus
Gastroprotective activity of ent-beyerene derivatives in mice: Effects on gastric secretion, endogenous prostaglandins and non-protein sulfhydryls
Li-7(BH)(5)(+): a new thermodynamically favored star-shaped molecule
Quercetin Affects Erythropoiesis and Heart Mitochondrial Function in Mice
Revisiting Aromaticity and Chemical Bonding of Fluorinated Benzene Derivatives
Seco-Taondiol, an Unusual Meroterpenoid from the Chilean Seaweed Stypopodium flabelliforme and Its Gastroprotective Effect in Mouse Model
Adaptive Responses of Mitochondria to Mild Copper Deprivation Involve Changes in Morphology, OXPHOS Remodeling and Bioenergetics
AHL signaling molecules with a large acyl chain enhance biofilm formation on sulfur and metal sulfides by the bioleaching bacterium Acidithiobacillus ferrooxidans
Assessment of elliptic flame front propagation characteristics of hydrogen in an optically accessible spark ignition engine
Copper deficiency alters cell bioenergetics and induces mitochondrial fusion through up-regulation of MFN2 and OPA1 in erythropoietic cells
Why is quercetin a better antioxidant than taxifolin? Theoretical study of mechanisms involving activated forms
The extremophile Acidithiobacillus ferrooxidans possesses a c-di-GMP signalling pathway that could play a significant role during bioleaching of minerals
AHL communication is a widespread phenomenon in biomining bacteria and seems to be involved in mineral-adhesion efficiency
Immune response in mice and cattle after immunization with a Boophilus microplus DNA vaccine containing bm86 gene
A theoretical analysis of codon adaptation index of the Boophilus microplus bm86 gene directed to the optimization of a DNA vaccine
In vivo binding of the Cry11Bb toxin of Bacillus thuringiensis subsp. medellin to the midgut of mosquito larvae (Diptera: Culicidae)
The injection of plasmid DNA in mouse muscle results in lifelong persistence of DNA, gene expression, and humoral response
Dexamethasone inhibits apoptosis of human neutrophils induced by reactive oxygen species

ConferencePaper (3)

C-di-GMP pathway in biomining bacteria
C-di-GMP pathway in acidithiobacillus ferrooxidans: Analysis of putative diguanylate cyclases (DGCs) and phosphodiesterases (PDEs) bifunctional proteins
Is the quorum sensing type AI-1 system of Acidithiobacillus ferrooxidans involved in its attachment to mineral surfaces?

Proyecto (2)

CORRELACION ENTRE LA DINAMICA MITOCONDRIAL Y EL REMODELAMIENTO Y ESTADO ENERGETICO DE LA OXPHOS EN MAMIFEROS.
Role Of Hig2a, Hig-1 Hypoxia Inducible Domain Family Member 2a, In The Regulation Of Mitochondrial Physiology

Review (1)

The Interplay among PINK1/PARKIN/Dj-1 Network during Mitochondrial Quality Control in Cancer Biology: Protein Interaction Analysis
33
Lina Ruiz

Academico Docente Investigador

Instituto de Ciencias Biomédicas

Universidad Autónoma de Chile

Santiago, Chile

8
William Tiznado

Full professor

Chemical Sciences

UNIVERSIDAD ANDRES BELLO

Santiago, Chile

5
Alvaro Elorza

ASSOCIATE PROFESSOR

CENTER FOR BIOMEDICAL RESEARCH

UNIVERSIDAD ANDRES BELLO

Santiago, Chile

4
Nicolas Simon Dominique Guiliani

Profesor Titular

Biología

UNIVERSIDAD DE CHILE

Santiago, Chile

3
Matias Castro

Investigador Asociado

Universidad de Chile

Santiago, Chile

3
Carlos Areche

ASSOCIATE PROFESSOR

CHEMISTRY

UNIVERSIDAD DE CHILE

Santiago, Chile

2
Mauricio González

Associate Professor

UNIVERSIDAD DE CHILE - INSTITUTO DE NUTRICION Y TECNOLOGIA DE ALIMENTOS

Santiago, Chile

2
Alex Gonzalez

Profesor Asociado

Ciencias Biologicas

Universidad de los Lagos

Osorno, Chile

2
Felipe Simon

Profesor Titular

Department of Biological Sciences

UNIVERSIDAD ANDRES BELLO FAC DE CS BIOLOGICAS, DPTO DE CS BIOLOGICAS

Santiago, Chile

2
Julio Benites

Profesor Titular

Química y Farmacia

Universidad Arturo Prat

Iquique, Chile

1
Rodrigo Quintanilla

Associate professor

Universidad Autónoma de Chile

Santiago, Chile

1
Luis Alvarez

UNIVERSIDAD ANDRES BELLO

Santiago, Chile

1
Mario Vera

Profesor Asistente

Instituto de Ingeniería Biológica y Medica

PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

Santiago, Chile

1
Alex Echeverria

Researcher

Vicerrectoría de investigación y postgrado

Universidad Católica del Maule

Talca, Chile

1
Mario Simirgiotis

Associate professor

Facultad de Ciencias

UNIVERSIDAD AUSTRAL DE CHILE

valdivia, Chile

1
Alvaro Gonzalez

Chief Technology Officer

Cellter

Santiago, Chile

1
Edwin Pérez

Profesor Asociado

Departamento de Química Orgánica

PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

Santiago, Chile

1
Rodolfo Paredes

Ungraduated General Director

Academic Vice Rectory

UNIVERDAD ANDRÉS BELLO

Santiago, Chile

1
Carlos Cardenas

Associate professor

Física

UNIVERSIDAD DE CHILE

Santiago, Chile

1
Patricio Armando Fuentealba

Profesor Titular

Física

U. DE CHILE, FAC. CIENCIAS, DPTO. FISICA

Santiago, Chile

1
Bruce Cassels

Profesor Titular

Departamento de Química

UNIVERSIDAD DE CHILE, FACULTAD DE CIENCIAS, DEPARTAMENTO DE QUÍMICA

Santiago, Chile

1
Gloria Cardenas

Full Professor

Department of Environmental Science

UNIVERSIDAD DE SANTIAGO DE CHILE

Santiago, Chile

1
Edison Osorio

UNIVERSIDAD ANDRES BELLO

Santiago, Chile

1
Cecilia Demergasso

Directora

Vicerrectoría de Investigación y Desarrollo Tecnológico

UNIVERSIDAD CATÓLICA DEL NORTE

Antofagasta, Chile

1
Marlén Barreto

Profesor asociado

UNIVERSIDAD AUTONOMA DE CHILE

Santiago de Chile, Chile

1
Claudia Riedel

Associate Professor

Ciencias Biológicas

Universidad Andres Bello

Santiago, Chile