Man

Maria Ines Becker Contreras

Scientific Director, President

FUNDACIÓN CIENCIA Y TECNOLOGÍA PARA EL DESARROLLLO

Santiago, Chile

Líneas de Investigación


Immunology: Mechanism of action of non-specific immunostimulants. Immunobiotechnology: Development of murine monoclonal antibodies.

Educación

  •  Immunobiotechnology, UNIVERSIDAD DE CHILE. Chile, 1989

Experiencia Académica

  •   Associate Professor Part Time

    UNIVERSIDAD DE CHILE

    Physical and Mathematical Sciences

    Santiago, Chile

    Enero 1999 - A la fecha

Experiencia Profesional

  •   Research director Full Time

    BIOSONDA S.A.

    Santiago, Chile

    Enero 1997 - A la fecha

  •   Research Director, Head of Immunology Laboratory Full Time

    Bios Chile Ingeniería Genética

    Santiago, Chile

    Enero 1989 - Enero 1996

Formación de Capital Humano


PREGRADE THESIS DIRECTION

1) Student:Viviana Cachicas
Professional Degree: Biochemist
Title Thesis: Estudio de fosfatasa alcalina durante el desarrollo inicial del ratón mediante anticuerpos monoclonales
University: Universidad de Concepción
Rol in Thesis: Co-Director with Luis Izquierdo and Alfredo De Ioannes
Begin Year: 1986
End Year: 1988

2) Student: Juan Aguayo
Professional Degree: Biochemist
Title Thesis: Producción de anticuerpos monoclonales contra las hormonas tiroideas T3 y T4
University: Universidad de Concepción
Rol in Thesis: Co-Director with Alfredo De Ioannes
Begin Year: 1990
End Year: 1991

3) Student: Andrea Giacaman
Professional Degree: Biochemist
Title Thesis: Obtención y caracterización de anticuerpos monoclonales contra componentes de la zona pelúcida de Hamster
University: Universidad de Concepción
Rol in Thesis: Co-Director with Alfredo De Ioannes
Begin Year: 1989
End Year: 1991

4) Student: Juan Jesús Elizalde
Professional Degree: Biochemist
Title Thesis: Estudio de la respuesta inmune humoral de salmonídeos, contra el agente causal del Síndrome del Salmón coho (Oncorhyncus kisutch)
University: Universidad de Chile
Rol in Thesis: Director
Begin Year: 1992
End Year: 1993

5) Student: Romy Berenguela
Professional Degree: Biochemist
Title Thesis: Desarrollo de un reactivo monoclonal de Coombs exclusivamente monoclonal
University: Universidad de Chile
Rol in Thesis: Director
Begin Year: 1993
End Year: 1994

6) Student: Caroll Beltrán
Professional Degree: Biochemist
Title Thesis: Desarrollo de un inmunoensayo para la detección de Histamina en harina de pescado
University: Universidad de Concepción
Rol in Thesis: Director
Begin Year: 1996
End Year:1997

7) Student: Headdy Manosalva
Professional Degree: Biochemist
Title Thesis: Producción de anticuerpos anti-idiotipos para la cuantificación de mollerosina en harina de pescado mediante ELISA de competencia”.
University: Universidad de Chile
Rol in Thesis: Director
Begin Year: 1997
End Year: 1998

8) Student: Harold Oliva
Professional Degree: Biochemist
Title Thesis: Caracterización inmunoquímica de la hemocianina del molusco Concholepas concholpeas
University: Pontificia Universidad Católica de Chile
Rol in Thesis: Director
Begin Year: 1999
End Year: 2000

9) Student: Leydi Lagos
Professional Degree: Biochemist
Title Thesis: Paricipación de células Natural Killer en el efecto inmunoestimulante de hemocianinas
University: Universidad de Chile
Rol in Thesis: Director
Begin Year: 2005
End Year: 2006

10) Student: Cecilia Espinoza
Professional Degree: Biochemist
Title Thesis: Caracterización bioquímica e inmunoquímica de la hemocianina de tres especies de lapas chilenas de la familia Fisurellidae: Fisurella latimarginata (Lapa negra), Fisurella cumingi (Lapa frutilla) y Fisurella maxima (Lapa reina
University: Pontificia Universidad Católica
Rol in Thesis: Director
Begin Year: 2006
End Year: 2007

11) Student: Miguel Del Campo
Professional Degree: Biochemist
Title Thesis: Efecto de hemocianinas de moluscos en la maduración de células dendríticas murinas
University: Universidad de Chile
Rol in Thesis: Director
Begin Year: 2006
End Year: 2007

12) Student: Andrea Gonzalez
Professional Degree: Biochemist
Title Thesis: Análisis mediante polarografía de la unión de oxígeno a hemocianina de Concholepas concholepas
University: Pontificia Universidad Católica de Chile
Rol in Thesis: Director
Begin Year: 2007
End Year: 2008

13) Student: Raimundo Born
Professional Degree: Molecular Biotechnology Engineer
Title Thesis: Participacion de macrofagos en el efecto inmunoestimulante de hemocianinas de moluscos en mamiferos
University: Universidad Chile
Rol in Thesis: Director
Begin Year: 2011
End Year: 2012

14) Student: Miriam Palacios
Professional Degree: Civil Engineer in Biotechnology
Title Thesis: Evaluación de hemocianinas (FLH, CCH y KLH) como carrier/adyuvante de antígenos tumorales
University: Universidad de Chile
Rol in Thesis: Director
Begin Year: 2013
End Year: 2014

MAGISTER THESIS DIRECTION

1) Student: Isabel Carrasco
Degree: MSc in Food
Title Thesis: Producción de anticuerpos de alta afinidad para la cuantificación de Mollerosina en harinas de pescado mediante ELISA
University: Universidad de Chile
Rol in Thesis: Director
Begin Year: 1995
End Year: 1996

2) Student: Fabián Salazar
Professional Degree: MSc in Biochemistry, specialisation in Proteins and Biotechnology
Title Thesis: Búsqueda de péptidos antimicrobianos en hemolinfa y hemocitos de especies de moluscos chilenos de interés productivo, incluyendo
Concholepas concholepas y especies de lapas del género Fissurellidae
University: Universidad de Chile
Rol in Thesis: Director
Begin Year: 2008
End Year: 2010

3) Student: Ta-Ying Zhong
Professional Degree: MSc in Biochemistry, specialisation in Proteins and Biotechnology
Title Thesis: Patrón de expresión de citoquinas y quimioquinas de células presentadoras de antígeno murinas en respuesta a estimulación in vitro
conhemocianinas de moluscos.
University: Universidad de Chile
Rol in Thesis: Director
Begin Year: 2012
End Year: 2014

DOCTORAL THESIS DIRECTION

4) Student: Sergio Arancibia
Professional Degree: PhD in Biochemist
Title Thesis: Participación de receptores tipo-lectina en el efecto antitumoral de hemocianinas.
University: Universidad de Chile
Rol in Thesis: Director
Begin Year: 2009
End Year: 2012

PARTICIPATION IN DOCTORAL PROGRAMS

- 2005 - present. Member of the Committee of Ph.D. in Biochemistry, Faculty of Science Chemical and Pharmaceutical, Universidad of Chile.


Premios y Distinciones

  •   45th Anniversary Faculty of Sciences, 2010. Ex-Student Award for his distinguished career in the scientific and entrepreneurship activities

    Universidad de Chile

    Chile, 2010

    45 th Anniversary Faculty of Science. University of Chile


 

Article (42)

Immunotherapeutic Potential of Mollusk Hemocyanins in Combination with Human Vaccine Adjuvants in Murine Models of Oral Cancer
TLR4, but Neither Dectin-1 nor Dectin-2, Participates in the Mollusk Hemocyanin-Induced Proinflammatory Effects in Antigen-Presenting Cells From Mammals
Antitumor activity and carrier properties of novel hemocyanins coupled to a mimotope of GD2 ganglioside
Immunomodulatory properties of Concholepas concholepas hemocyanin against francisellosis in a zebrafish model
Molluskan Hemocyanins Activate the Classical Pathway of the Human Complement System through Natural Antibodies
The oxygen-binding properties of hemocyanin from the mollusk Concholepas concholepas
Hemocyanins stimulate innate immunity by inducing different temporal patterns of proinflammatory cytokine expression in macrophages
Oxidative Modifications in Crystallin Proteins and Lens Epithelial Cells Associated with Photosensitized Reactions Mediated by the Major Chromophore Arising from Glucose Degradation
Immunocompetence of breeding females is sensitive to cortisol levels but not to communal rearing in the degu (Octodon degus)
A Novel Immunomodulatory Hemocyanin from the Limpet Fissurella latimarginata Promotes Potent Anti-Tumor Activity in Melanoma
Antiproliferative and Uncoupling Effects of Delocalized, Lipophilic, Cationic Gallic Acid Derivatives on Cancer Cell Lines. Validation in Vivo in Singenic Mice
Changes in the expression pattern of developmentally important genes and possible relation to the low in vitro and in vivo developmental potential of transgenic nuclear transfer embryos
Tumour cell lysate-loaded dendritic cell vaccine induces biochemical and memory immune response in castration-resistant prostate cancer patients
Enhanced structural stability of Concholepas hemocyanin increases its immunogenicity and maintains its non-specific immunostimulatory effects
Novel hemocyanin from the Fissurella latimarginata exhibits an outstanding immunogenicity and non-specific immunomodulatory effects in a melanoma model
Different cell death mechanisms are induced by a hydrophobic flavin in human tumor cells after visible light irradiation
Hemocyanins as immunostimulants Hemocianinas, una herramienta inmunológica de la biomedicina actual
Concholepas hemocyanin biosynthesis takes place in the hepatopancreas, with hemocytes being involved in its metabolism
Chemosensitizing Effect of Nordihydroguaiaretic Acid and its Tetra-acetylated Derivative on Parental and Multiresistant TA3 Mouse Mammary Adenocarcinoma Cells
Cutting Edge: Stealth influenza Virus Replication Precedes the Initiation of Adaptive Immunity
Immunodominant role of CCHA subunit of Concholepas hemocyanin is associated with unique biochemical properties
Inhibitory effect of nordihydroguaiaretic acid and its tetra-acetylated derivative on repiration and growth of adenocarcinoma TA3 and its multiresistant variant TA3MTX-R
Females of the communally breeding rodent, Octodon degus, transfer antibodies to their offspring during pregnancy and lactation
Immunotherapeutic effect of concholepas hemocyanin in the murine bladder cancer model: Evidence for conserved antitumor properties among hemocyanins
SURFACE ENHANCED RAMAN SPECTRUM OF NANOMETRIC MOLECULAR SYSTEMS
Anti-idiotype gizzerosine 3A12 (anti-idiotype GZ 3A12)
Hemocyanin of the molluscan Concholepas concholepas exhibits an unusual heterodecameric array of subunits
Anti-Concholepas concholepas hemocyanin (anti-CCH 1A4)
Anti-Concholepas concholepas hemocyanin (anti-CCH 1E4)
Anti-Concholepas concholepas hemocyanin (anti-CCH 1E8)
Anti-Concholepas concholepas hemocyanin (anti-CCH 1H5)
Anti-Concholepas concholepas hemocyanin (anti-CCH 2A8)
Anti-Concholepas concholepas hemocyanin (anti-CCH 2H10)
Anti-Concholepas concholepas hemocyanin (anti-CCH 3A5)
Anti-Concholepas concholepas hemocyanin (anti-CCH 3B7)
Anti-Concholepas concholepas hemocyanin (anti-CCH 3E3)
Anti-Concholepas concholepas hemocyanin (anti-CCH 4E8)
Anti-Concholepas concholepas hemocyanin (anti-CCH 7B4)
Anti-Concholepas concholepas hemocyanin (anti-CCH 8E5)
Monoclonal antibodies to molluskan hemocyanin from Concholepas concholepas demonstrate common and specific epitopes among subunits
Characteristics of monoclonal antibodies against Piscirickettsia salmonis
Reactivity of monoclonal antibodies against a tryptophan-riboflavin adduct toward irradiated and non-irradiated bovine-eye-lens protein fractions: an indicator of in vivo visible-light-mediated phototransformations

Proyecto (10)

Adquisición de un sistema de biorreactor para el escalamiento de la producción de biomasa y compuestos de interés biotecnológico a partir de células animales en cultivo
Mollusk hemocyanins as immunomodulatory proteins with beneficial clinical outcomes: Elucidating the role of C-type lectin receptors in these effects.
Identificacion de peptidos antimicrobianos desde moluscos litorales chilenos y potencial uso biotecnologico
Immunological basis involved in the immunomodulatory effects of mollusk hemocyanins in the immune system of mammals
Péptidos bioactivos de hemocianina de moluscos chilenos, con aplicaciones biotecnologicas
Desarrollo de productos inmunoestimulantes en base a hemocianina de C. concholepas
Estudio de la hemocianina de moluscos en relación a sus mecanismos de inmunoestimulación en mamíferos.
Investigación y desarrollo de terapias biológicas optimizadas para tumores urológicos
Desarrollo de un proceso para producir subunidades de hemocianina para formular inmunoestimulantes de alta potencia.
Evaluación de las propiedades inmunoestimulantes de la hemocianina de loco (Concholepas concholepas): Establecimiento de un modelo de estudio y de su posible mecanismo de acción.

Abstract (5)

Exceptional Immunological and Anticancer Properties of a New Hemocyanin from Fissurella Latimarginata (FLH)
Antitumor Effect of Concholepas Concholepas Hemocyanin (CCH) Using B16-F10 Mouse Melanoma Cells as a Model
Biochemical and Immunomodulatory Properties From Concholepas Hemocyanin (CCH) and Their Isolated Subunits
CHEMOSENSITIZING EFFECT OF NORDIHYDROGUAIARETIC ACID (NDGA) AND ITS TETRA ACETYLATED DERIVATIVE (NDGATA) ON PARENTAL AND MULTIRESISTANT TA3 MOUSE MAMMARY ADENOCARCINOMA CELL LINES
Cryopreservation and immunoreactivity of proacrosin/acrosin system in alpaca spermatozoa.

Patent (4)

Extracto de hemocitos de Loco (Concholepas concholepas) con actividad antimicrobiana, composición, procedimiento de preparación y sus usos.
Hemocianina purificada, sus subunidades o sus fragmentos, obtenidos desde la hemolinfa de Fissurella latimarginata, Fissurella cumingi y Fissurella maxima.
Purified hemocyanin obtained from Fissurella latimarginata, Fissurella cumingi or Fissurella maxima; subunit of purified hemocyann; use of hemocyanin, its subunits or immunogenic fragments and compositions containing the same.
Product and composition containing a Concholepas concholepas hemocyanin (CCH) subunit, and a method of use thereof.

BookSection (2)

Hemocyanins as Natural Immunostimulants in Biomedical Applications
Hemocyanins in the immunotherapy of superficial bladder cancer.
63
Maria Becker

Scientific Director, President

Research and Development

FUNDACIÓN CIENCIA Y TECNOLOGÍA PARA EL DESARROLLLO

Santiago, Chile

10
Augusto Manubens

Director

Tecnical Support

BIOSONDA S.A.

Santiago, Chile

8
Miguel del Campo

Investigador Adjunto Proyecto FONDECYT 151337

Fundación Ciencia y Tecnología para el Desarrollo

Santiago, Chile

6
Jorge Ferreira

Director

Clinic and Molecular Farmacology

UNIVERSIDAD DE CHILE , FACULTAD DE MEDICINA, ICBM

Santiago, Chile

4
Sergio Arancibia

Head of Vaccine Development

Investigación y Desarrollo

Virbac

Santiago, Chile

3
Juan Maya

Associated Proffesor

Instituto de Ciencias Biomedicas

UNIVERSIDAD DE CHILE

Santiago, Chile

3
Lorena Aguilar

Assistant Professor

Pathology

UNIVERSIDAD DE CHILE, FACULTAD DE CIENCIAS VETERINARIAS Y PECUARIAS

Santiago, Chile

2
Ramiro Araya

UNIVERSIDAD DE CHILE, FAC. CIENCIAS QUÍMICAS Y FARMACÉUTICAS, DPTO. QUÍMICA ORGÁNICA Y FISICOQUÍMICA

Santiago, Chile

2
Patricio Leyton

Profesor Asociado

INSTITUTO DE QUÍMICA

PONTIFICIA UNIVERSIDAD CATÓLICA DE VALPARAÍSO

Valparaíso, Chile

2
Ana Edwards

Full Professor

Chemistry

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE

Santiago, Chile

1
Ulrike Kemmerling

Associate Professor

ICBM

Facultad de Medicina, Universidad de Chile

Santiago, Chile

1
Eduardo Parra

ACADÉMICO - ESCUELA DE MEDICINA

MEDICINA

UNIVERSIDAD DE TARAPACA

Arica, Chile

1
Mercedes Lopez

Profesora Asociada

Instituto de Ciencias Biomédicas

Universidad de chile

Santiago, Chile

1
Sebastian Abades

Director

Universidad Mayor

Santiago, Chile

1
Alvaro Lladser

Associate Researcher / Principal Investigator

FUNDACIÓN CIENCIA PARA LA VIDA

Santiago, Chile

1
Raul Valdevenito

Chair

UNIVERSIDAD DE CHILE, HOSPITAL CLINICO UNIVERSIDAD DE CHILE

Santiago, Chile

1
Enrique Castellon

Profesor Titular. Director

Oncología Básico-Clínica

UNIVERSIDAD DE CHILE

Santiago, Chile

1
Roberto Ebensperger

Associate Professor

Pharmacy

Pontificia Universidad Católica de Chile

Santiago, Chile

1
Lorena Salazar

Academico

Instituto Cs Biomédicas

Universidad Autónoma de Chile

Santiago, Chile

1
Alejandro Venegas

Profesor Titular asociado al Centro

Universidad Diego Portales

Santiago, Chile

1
Mario Vera

Profesor Asistente

Instituto de Ingeniería Biológica y Medica

PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

Santiago, Chile

1
José Jara

Assistant Professor

Research Institute of Dental Sciences

Universidad de Chile

Santiago, Chile

1
ARTURO FERREIRA

FULL PROFESSOR, CHAIR IMMUNOLOGY DISCIPLINARY PROGRAM

IMMUNOLOGY

UNIVERSIDAD DE CHILE

SANTIAGO, Chile

1
Jaime Figueroa

UNIVERSIDAD AUSTRAL DE CHILE

Valdivia, Chile

1
Luis Burzio

Scientific Director

Andes Biotechnologies

FUNDACION CIENCIA PARA LA VIDA

Santiago, Chile

1
Fabian Salazar

Postdoctoral Research Fellow

Institute of Medical Sciences

MRC Centre for Medical Mycology, University of Aberdeen

Aberdeen, Reino Unido

1
Lilian Jara

Titular Professor

Human Molecular Genetic

UNIVERSIDAD DE CHILE / FACULTAD DE MEDICINA

Santiago, Chile

1
Pablo Valenzuela

Founder and Scientific Director

Fundación Ciencia & Vida

Santiago, Chile