Neurobiology, Cancer, Stem Cells, Death Cell, Vitamin C, Glucose and Vitamin C Transporters, aging, Choroid Plexus Cells Biology

Formation of Human Capital

Undergraduate

1. Alejandro Godoy."Characterization of glucose transporters in cancer". Medical Technologist Undergraduate, Frontera University. 1995. Tutor

2. Claudio Aguayo."Characterization of glucose transporters in human brain". Medical Technologist Undergraduate, Frontera University. 1995. Tutor

3. Carolina Balmaceda. "Immunolocalization and molecular characterization of the glucose transporter GLUT 1 in cartilaginous fish Scyliorhinus canicula and Schroedericthys chilensis and bony fish Oncorhynchus mykiss brains." Biologist Thesis. Concepción University. 2001. Tutor

4. Sofía Barberis."Hexose transporters expression in human prostate cancer". Biochemistry Undergraduate Thesis. Concepción University. 2000. Co-Tutor

5. Mónica Carrasco."GLUT1 localization and expression analysis in normal and neoplastic ependymal glia". Biochemistry Undergraduate Thesis. Concepción University. 2001. Tutor

6. Maite Castro. "Characterization of the transport of vitamin C in cells of the central nervous system". Biochemistry undergraduate Thesis. Concepción University. 2000. Tutor

7. Carola Millán."OSC-spondin: Analysis of expression in the subcommissural organ and partial sequencing of its mRNA in the rat OSC". Biochemistry Undergraduate Thesis. Austral University of Chile. 2002. Co-tutor. Co-Tutor

8. Claudio Morales. "Hexose transporters analysis in human malignant melanoma". Biochemistry Undergraduate Thesis. Concepción University. 2002. Tutor

9. Viviana Ulloa."Characterization of the transport of vitamin C in human tumor cells of the choroid plexus". Pharmacist Undergraduate Thesis. Concepción University. 2003. Co-Tutor

10. Patricia Pastor."Postnatal neurogenic niche differentiation, localization of the precursor cells (stem cells) in mouse and human." Biochemistry Undergraduate Thesis. Concepción University. 2003. Tutor

11. Carmen Silva. "Cellular markers in radial glia differentiation". Biochemistry Undergraduate Thesis. Concepción University. 2004. Tutor

12. Christian Cortés Campos. "MCT1 and MCT2 expression and localization in glial cells of the hypothalamus." Biochemistry Undergraduate Thesis. Concepción University. 2005. Co-Tutor

13. Marcela Low M."SVCT1 and SVCT2 polarization in kidney cells." Biochemistry Undergraduate Thesis. Concepción University. 2004. Tutor

14. Katterine Salazar."SVCT2 distribution in neurons and glial cells ". Biochemistry Undergraduate Thesis. Concepción University. 2003. Tutor

15. Pedro Cisternas F. "Study of Vitamin C recycling between astrocytes and neurons". Biochemistry Undergraduate Thesis. Concepción University. 2006. Co-Tutor

16. Karina Oyarce. “Vitamin C and neuronal differentiation”. Bioingeniering undergraduate Thesis. Concepción University. 2009 - 2010. Tutor
17. Nery Jara. “Neurogenesis and brain tumors”. Biochemistry Undergraduate Thesis. Concepción University. 2009 - 2010. Tutor

18. Gustavo Cerda. "Lentiviral vectors for SVCT2 overexpression". Bioingeniering Undergraduate Thesis. Concepción University. 2013. Co-Tutor

19. Luciano Ferrada. "Vitamin recycling between astrocytes and neurons and death induction". Bioingeniering Undergraduate Thesis. Concepción University. 2013. Tutor

20. Francisca Espinoza. "Effect of retinoic acid and ascorbic acid on the SVCT2 expression in neural stem cells". Biochemistry Undergraduate Thesis. Concepción University. 2015. Tutor

21. Victor Baeza. "Characterization of IIIG9 expression in ependymal cells". Biochemistry Undergraduate Thesis. Concepción University. (2015). Co-Tutor

22. María José Oviedo. "Analysis of the expression, localization and interaction of the isoforms of the protein phosphatase 1 (PPP1) and the ciliary protein IIIG9 in ependymal cells". Biochemistry Undergraduate Thesis. Concepción University. 2017. Co-Tutor

23. Rocío Magdalena Parra. "Effect of dehydroascorbic acid on neuronal metabolism". Biochemistry Undergraduate Thesis. Concepción University. (2019). Tutor

24. Cristian Sanzana. "Vitamin C transporters recycling in aging". Bioingeniering Undergraduate Thesis. Concepción University. (2020). Tutor

25. Ninoska Troncoso. "Bystander effect and ferroptosis induction". Bioingeniering Undergraduate Thesis. Concepción University. (2020). Tutor

Post-graduate

Master

1. Alejandro Godoy. "Expression and function of glucose transporters in cancer, with special emphasis on breast cancer cells". Master Thesis, Concepción University. Tutor. 2000.

2. Federico Rodríguez. "Vitamin C uptake in human glioma cells: Application of a model of resistance to cytostatic therapies". Master Thesis, Concepción University. Tutor. 2000.

3. Roberto Elizondo. Differential characterization of the ability to release lactate by cortical and hypothalamic glial cells. Master Thesis, Concepción University. Co-Tutor. 2010.

4. Paula Llanos. Inhibition of GLUT2 in hypothalamic tanycytes and its effect on food intake in vivo. Master Thesis, Concepción University. Co-Tutor. 2013.

5. 25. Cristian Sanzana. "Vitamin C effects in aging and brain cells death ". Master Thesis, Concepcion University. Tutor. 2020

PhD Thesis

1. María García. "Study of the expression and function of hexose transporters and vitamin C in normal and tumor ependymal cells". PhD Thesis. Cellular and Molecular Biology Program. Concepcion University. 2001. Tutor.

2. Maite Castro. "Effect of vitamin C on glucose uptake and accumulation in neurons of the cerebral cortex". PhD Thesis. Cellular and Molecular Biology Program. Austral University of Chile. 2005. Tutors, Dr. Francisco Nualart, Dra. Ilona Concha.

3. Allisson Astuya. "Uptake and intracellular action of vitamin C in developing neurons and human neuroblastoma cell lines". PhD Thesis. Cellular and Molecular Biology Program. Concepcion University. 2006. Tutor.

4. Tamara Castro. "Dynamic and polarized expression of Vitamin C transporters in kidney epithelial cells". PhD Thesis. Cellular and Molecular Biology Program. Concepcion University. 2007. Tutor.

5. Carola Millán."Localization, regulation and inhibition of glucokinase in the hypothalamic glia and its regulation in eating behavior". PhD Thesis. Cellular and Molecular Biology Program. Concepcion University. 2008. Tutor.

6. Carolina Balmaceda. "Cellular and molecular bases for explaining the phylogenetically model of glia-neuron metabolic coupling". PhD Thesis. Cellular and Molecular Biology Program. Concepcion University. 2009. Tutor.

7. Viviana Ulloa."Looking for the molecular and functional bases to explain the entry of vitamin C into the brain through the choroid plexus." PhD Thesis. Cellular and Molecular Biology Program. Concepcion University. 2009. Tutor.

8. Carmen Silva A."Effect of vitamin C and and factors released from the meninges into the in vitro differentiation of radial glia."PhD Thesis. Cellular and Molecular Biology Program. Concepcion University. 2009. Tutor.

9. Katterine Salazar. “Vitamin C effect in neuronal differentiation, an in vitro approach”. PhD Thesis. Cellular and Molecular Biology Program. Concepcion University. 2010. Tutor.

10. Patricia Pastor. “Neurogenic niche and SVCT2 expression”. PhD Thesis. Cellular and Molecular Biology Program. Concepcion University. 2010. Tutor.

11. Christian Cortés. “MCTs participation in the glucose sensing mechanism based in the metabolic coupling glia-neuron”. PhD Thesis. Cellular and Molecular Biology Program. Concepcion University. 2011. Co-Tutors, M.A. García, F. Nualart.

12. Fernando Martínez. “Glucose sensing mechanism in hypothalamic glial cells”. PhD Thesis. Cellular and Molecular Biology Program. Concepcion University. 2012. Co-Tutors, MA. García, F. Nualart.

13. Marcela Low. “SVCT2 expression and vitamin C function in neuromuscular differentiation”. PhD Thesis. Cellular and Molecular Biology Program. Concepcion University. 2010. Co-Tutors, JP Henríquez, F. Nualart.

14. Zahady Velásquez. “Localization and function of Fructose-1,6-bifosfatase rat brain”. PhD Thesis. Cellular and Molecular Biology Program. Austral University. 2011. Co-Tutors, JC Slebe, F. Nualart.

15. Pedro Cisternas. “Vitamin C recycling and glutamate effect in astrocytes”. PhD Thesis. Cellular and Molecular Biology Program. Concepcion University. 2012. Tutor.

16. Andrea García. “Oxidized vitamin C affects NFkB activation in neurons”. PhD Thesis. Cellular and Molecular Biology Program. Concepcion University. 2012. Tutor.

17. Daniel Sandoval. “Subcellular localization and SVCT2 functional activity in skeletal muscle cells”. PhD Thesis. Cellular and Molecular Biology Program. Concepcion University. 2012. Co-Tutors, J.P. Henríquez, F. Nualart.

18. Karina Oyarce. “Effect of vitamin C in neuronal differentiation”. PhD Thesis. Cellular and Molecular Biology Program. Concepcion University. 2015. Tutor.

19. Nery Jara. “Neurogenesis and their effect on tumor growth”. PhD Thesis. Cellular and Molecular Biology Program. Concepcion University. 2016. Tutor.

20. Francisca Espinoza. "Effect of vitamin C recycling in the neurites formation". PhD Thesis. Cellular and Molecular Biology Program. Concepcion University. (In progress). Tutor.

21. Eder Ramírez. "Functional role of vitamin C in glioblastoma progression". PhD Thesis. Cellular and Molecular Biology Program. Concepcion University. 2020. Tutor.

22. Natalia Saldivia. "Effect of vitamin C on the postnatal radial glia of normal brains and slc23a2 +/-. PhD Thesis. Cellular and Molecular Biology Program. Concepcion University. 2017 (In progress). Tutor.

23. Víctor Baeza. "Study of the expression and function of IIIG9 in the differentiation and polarization of normal and tumor ependymal cells". PhD Thesis. Cellular and Molecular Biology Program. Concepcion University. 2017 (In progress). Co-Tutor.

24. Luciano Ferrada. "Necroptosis in neuronal cells under oxidative stress". PhD Thesis. Cellular and Molecular Biology Program. Concepcion University. 2018. Tutor.

25. Rocío Magdalena. "RIPK1 functional regulation by Dehydroascorbic acid". PhD Thesis. Cellular and Molecular Biology Program. Concepcion University. 2020 (In progress). Tutor.

Post Docs

Dr. Viviana Ulloa, Supported by Innova Bio Bio Grant.
Dr. Katherin Forman, Supported by Fondecyt Grant.
Dr. Romina Bertinat, Supported by Fondecyt Grant.
Dr. Carolina Balmaceda. Assistant Professor UDD.
Dr. Fernando Martínez. Supported by Innova Bio Bio Grant.
Dr. María José Barahona, Supported by Fondecyt Grant.

- CMA Brochure. January 2012, CMA Bío-Bío produces a brochure with information about the center.
- Biomedical Science. San Sebastian University invites the CMA to publish about the Center in its scientific journal. This magazine is distributed from Arica to Puerto Montt.
- Biological Sciences newsletter "In Touch". Volume 12 (1) July 2012.
Center for Advanced Microscopy Bio-Bio: a pole of services with application
technological, scientific and industrial of the highest level in the country.
- Chilean Society of Biochemistry and Molecular Biology. Web page
http://www.microscopy.info advertises the CMA as a microscope facility in Chile.
- Aquaculture "Technical Magazine Different Version, The magazine is distributed in 4000 copies to all Chilean Salmon farms, Crops Centers, Hatcheries, Process plants.
I D i Magazine University of Concepción. May 3, 2013 Inauguration of the CMA
Bio-Bio.
- In 2012, it participated in 3 radio programs of the University to discuss aspects related to Research, Post-Graduate Training and Research Projects and Microscopy.
- Talk "Confocal Laser Microscopy". Collegiate Institute of Humanities, 3rd and 4th media. Advanced Microscopy Center, University of Concepción. August 12, 2014.
- Science Day October 4, 2014. Advanced Microscopy Center. CMA BIO BIO. The Center was kept open for external visitors to the University.
- Different Version Magazine. Advanced Microscopy Center. Application in Salmon and Aquaculture. Number 20, 2014.
- Revista I D i UDEC, December 2013. Woman with first transplant of differentiated stem cells, tells her new life.
- Talk "Confocal Laser Microscopy". Collegiate Institute of Humanities, 3rd and 4th media. Advanced Microscopy Center, University of Concepción. August 12, 2014.
- University Radio radio program, information is prepared for 2015.
- Website Fac. Biological Sciences, UDEC, Center for Advanced Microscopy Biobío, a center of excellence in the service of research. Tuesday, July 7, 2015. http://csbiolmas.blogspot.com/2015/07/centro-de-microscopia-avanzada-biobio.html.
- El Mercurio journal. Special editions. "Postgraduate Supplement in the South". Leaders in Confocal Microscopy. Sunday, July 12, 2015.
- EL MERCURIO. Special editions "Postgraduate Supplement in the South". CMA BIO BIO. Referent in light microscopy technologies. Sunday, July 12, 2015.
- UDEC Panorama. Article. Advanced Microscopy Center receives excellence evaluation. May 2016, No. 722. Page 16.
- Magazine I+D+ i UDEC. Insert on the renovation of CMA BIO BIO. P. 79. No 34, September 2016.
- Magazine PANORAMA UDEC, Advanced Microscopy Center received excellence evaluation. May, 2016, pag. 16
- The latest news. 3D images show how the most aggressive tumors are fed. Wednesday, August 30, 2017.
- Newspaper EL MERCURIO. CMA BIO BIO promotes strategy to open its services to new companies. March 11, 2018
- Conference Inauguration of the 2019 school year, Concepción, Chile. The different types of cell death, an inevitable fate.
- F. Nualart. Conference “Applied Microscopy to the analysis of biological functions. Antioxidative defense and cell death ”. Department of Microbiology, University of Concepción. April 2, 2019.
- Newspaper EL MERCURIO. CMA BIO BIO continues to contribute to development and knowledge at the national level. Wednesday June 12, 2019. Publication in El Mercurio, a national newspaper, with an inserted page about the CMA BIOBIO and its motivation for strategies to open services to new companies.
- Magazine PANORAMA UDEC, Researcher at the CMA publishes research on diabetes in a high-impact journal. August 14, 2019.
- Magazine PANORAMA UDEC, Talks organized by CMA Biobio were attended by specialists from the University of Oxford. October 28, 2019.
- NATIONAL TELEVISION PROGRAM. Recording in CMA BIOBIO for the TVN program Explorers, which includes three mini-reports, which substantiate the existence of the CMA in the sense of promoting the creativity of researchers with the development of methodologies that are only possible to carry out in the CMA BIOBIO (in Chile). The mini-reports addressed the advantages of different technologies available at the Center, making their applications visible for the research of Dr. Luciano Ferrada and the FlowInmunocell company; Dr. Romina Bertinat and Katterine Salazar and finally, Drs. Rosario Castillo and Juan Araya. This strategy has multiple objectives. On the one hand, mini-reports are focused on young researchers, showing the CMA as a niche that welcomes and enhances the scientific career. On the other hand, the Center's link with the business sector is strengthened, showing the experience of the agreement between FlowInmunocell and the CMA BIOBIO. The mini-reports showed the relevance of INCUCYTE S3 technologies, flow cytometry, spectral confocality and super resolution, and FT-IR microscopy. June 18-19, 2019. https://www.24horas.cl/programas/exploradores/exploradores-2019---miercoles-4-de-dic Diciembre- 3772827.
- Newspaper STRATEGY . CMA of the University of Concepción launches a laser technology microscope. http://www.diarioestrategia.cl/texto-diario/mostrar/1523453/cma-concepcion-estrena-microoscopiotecnologia-laser-ultima-generacion. September 12, 2019.
- Newspaper EL CONCECUENTE. SOCIETY section. Last generation microscopes with laser technology debut in Concepción. http://www.elconcecuente.cl/noticia/sociedad/en-concepcion-estrenan-microoscopio-de-ultimageneracion- con-tecnologia-laser. March 9, 2019.
- Newspaper EL SUR . Laser Microscopy will allow optimization of research at UDEC. https://www.elsur.cl/impresa/2019/09/12/full/cuerpo-principal/12/. 12 September 2019.
- Magazine PANORAMA UDEC. Highlights the level of course offered by the CMA BIOBIO, for having specialists from prestigious Universities such as the University of Oxford and the Max Planck Institute. It should be noted that the talks offered in the course organized by the CMA are very similar to those offered by the microscopy course taught at the University of Oxford. Link: http://www.udec.cl/ panoramaweb2016 / content / charlas-organized-por-cma-biob% C3% ADo-counted-with-presence-deespecialistas- de-la-universidad. October 29, 2019.
- NATIONAL TELEVISION PROGRAM. On December 9, 2019, the episode of the TVN Canal24Horas television program "Explorers: From Atom to Cosmos" was broadcast live. It had the direct participation of the Scientific Director of the Center, Dr. Francisco Nualart, who commented on basic aspects of microscopy and gave some indicators of the impact of CMA BIOBIO on national scientific work. https://www.24horas.cl/programas/exploradores/exploradores-2019---miercoles-4-de-dic Diciembre-3772827
- Redesign and construction of a new CMA BIO BIO WEB page (www.CMABIOBIO.cl). 2020
- Creation and implementation of the NEUROCELLT laboratory WEB page (www.neurocellt.cl). 02-06-2020
- Publication of Opinion Column on the website of the Faculty of Biological Sciences, UdeC. It can be reviewed at the following link https://cienciasbiologicasudec.cl/cma-en-retrospectiva. 03-06-2020.
- Activities of the Center for Advanced Microscopy 10 years after its creation (Link: https://noticias.udec.cl/centro-de-microscopia-avanzada-en-retrospectiva/. 05-06-2020.
- Newspaper THE LATEST NEWS. Research linked to CMA BIOBIO on cerebrovascular accidents. Publication in Redox Biology, impact WOS-ISI 10. 08-18-2020.
- VRID news page UDEC STUDY DETERMINES HOW NEURONAL DEATH OCCURS IN A BRAIN INFARCTION (http://vrid.udec.cl/?q=node/1404) 08-18-2020.
- News page of the University of Concepción (https://noticias.udec.cl/estudio-determina-como-seproduce-la-muerte-neuronal-en-un-infarto-cerebral/). 08-19-2020.
- Newspaper EL MOSTRADOR (https://www.elmostrador.cl/agenda-pais/2020/08/18/investigadores-descubren-quela- vitamin-c-triggers-neuronal-death-in-conditions-of-stress- oxidative /). 20-08-2020.
- August Newsletter of the Faculty of Biological Sciences (https://cienciasbiologicasudec.cl/wp-content/uploads/2020/08/newagosto.pdf). 08-30-2020.
- Newspaper cover Concepción. Researchers shed new light on strokes. (https://assets.diarioconcepcion.cl/2020/10/29-10-2020.pdf). 10-29-2020.
- Interview conducted by the Chilean Biology Society to be published on the Society's website, due to a scientific publication on cerebral infarction and neuronal death. 11-13-20. https://www.biologiachile.cl/2020/11/26/entrevista-dr-nualart-el-rol-dual-de-la-vitamina-c-en-la-muerte-neuronal/
- News Letter from the Faculty of Biological Sciences of the University of Concepción, as part of the promotion and enhanced linkage that has been fostered during the pandemic. This publication highlights vital aspects of the Center's work, such as its history, human team, objectives and some indicators such as papers, projects, etc. (https://cienciasbiologicasudec.cl/wp-content/uploads/2021/01/pdf). 10-12-2020.