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Dolores Busso Perkins

Associate Professor

Pontificia Universidad Católica de Chile. Escuela de Medicina

Santiago, Chile

Líneas de Investigación


Our work is aimed at understanding how metabolic and nutritional imbalances influence reproduction. Specifically, we study the contribution of cholesterol and vitamin E to female fertility, embryonic development and pregnancy outcome.

Educación

  •  Science, Universidad de Buenos Aires. Argentina, 1998
  •  Physiology, Universidad de Buenos Aires. Argentina, 2005

Experiencia Académica

  •   Assistant Investigator Full Time

    PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

    Santiago, Chile

    Enero 2010 - Enero 2012

  •   PostDoc Full Time

    PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

    Faculty of Biological Sciences

    Santiago, Chile

    Enero 2005 - Enero 2009

  •   PhD Student Full Time

    Instituto de Biología y Medicina Experimental - Universidad de Buenos Aires

    Buenos Aires, Argentina

    Enero 1998 - Enero 2005

  •   Fellow Full Time

    Institut de la Sante et Researche Medicale

    Paris, Francia

    Enero 1997 - Enero 1997

  •   Undergraduate Fellow Part Time

    Centro de Educación Médica e Investigaciones Clínicas

    Buenos Aires, Argentina

    Enero 1996 - Enero 1996

  •   Assistant Professor Full Time

    PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

    Faculty of Medicine

    Santiago, Chile

    Enero 2013 - Enero 2016

  •   Associate Professor Full Time

    PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

    Faculty of Medicine

    Santiago, Chile

    Enero 2016 - A la fecha

  •   Associate Professor Full Time

    PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

    Faculty of Medicine

    Santiago, Chile

    Enero 2016 - A la fecha

Formación de Capital Humano


Undergraduate Research Units

2006-2008. Mentor Biological Sciences student María José Oñate-Alvarado.
2009. Co-Mentor Biological Sciences student María José Marin.
2009. Co-Mentor Biological Sciences student Sebastian Pereda.
2010. Mentor Biochemistry student Nicolás Santander.
2011-2012. Mentor Medicine student Lilian Mascareño.
2012. Mentor Medicine student Isabella Passalaqua.
2012. Mentor Biological Sciences student Linda Quilaqueo.
2014 -2015. Mentor Biochemistry student Paz Molina.
2014 - 2016. Mentor Medicine student Andrés Schneider.
2016. Mentor Medicine student Daniel Gallardo.
2018. Mentor Biochemistry student Maximiliano Villarreal.
2019. Mentor Nutrition students Perina Kutulas and Magdalena Gutiérrez.

Undergraduate thesis

2011-2012. Thesis in Biochemistry. Nicolás Santander.
2013 - 2014. Thesis in Medical Technology. Constanza Andrea Baeza y Nathaly De La Jara
2013 - 2015. Thesis in Biochemistry. Alonso Quiroz.
2016 - 2017. Thesis in Biochemistry. Paz Molina.
2019. Thesis in Medical Technology. José Chavez.
2019. Thesis in Medical Technology. Nicolás Gonzalez.

Mentoring of international students

2011. MISTI-PUC Grant Fellowship. Allison Hamilos, from MIT (US)
2012. PLISSER Fellowship (Programa de Investigaciones en Salud Sexual y Reproductiva, Asociación Latinoamericana de Investigadores en Reproducción Humana). Natalia Pascuali, Universidad de Buenos Aires (Argentina)
2014. PLISSER Fellowship (Programa de Investigaciones en Salud Sexual y Reproductiva, Asociación Latinoamericana de Investigadores en Reproducción Humana). Paula Accialini Universidad de Buenos Aires (Argentina)
2015. MISTI-PUC Grant Fellowship. Dalia Walzer, from MIT (US)

Graduate Students

2012. Research Unit. Ph.D. Programme in Medical Sciences, School of Medicine. María José Parga.
2013. Research Unit. Ph.D. Programme in Medical Sciences, School of Medicine. Nicolás Santander.
2015. Research Unit. Ph.D. Programme in Medical Sciences, School of Medicine. Loni Berlowitz.

Masters Thesis

2012-2013. Co-Mentor. Master in Nutrition. School of Medicine. Grettel Martelo.
2016. Mentor. Master in Nutrition. School of Medicine. Marcela Riquelme.
2017. Mentor. Master in Nutrition. School of Medicine. Andrea David.

Ph.D. Thesis

2013 - 2016. Mentor. Ph.D. Programme in Physiology. School of Biology. Francisca Salas.
2013 - 2016. Co-Mentor. Ph.D. Programme in Medical Sciences, School of Medicine. Susana Contreras.
2014 - 2018. Mentor. Ph.D. Programme in Medical Sciences, School of Medicine. Nicolás Santander.
2017 - present. Mentor. Ph.D. Programme in Medical Sciences, School of Medicine. Alonso Quiroz.
2019 - present. Mentor. Ph.D. Programme in Biology (Physiology). School of Biology. Andreina Arias.


Difusión y Transferencia


MAIN OUTREACH ACTIVITIES

2007-2014. Member of the Board of Directors of the Chilean Society for Reproduction and Development: 2007-2008 (Treasurer), 2009-2012 (Director), 2012-2014 (Secretary)
2014-2015. Member of the Scientific Committee VI Congress Latin American Society of Maternal-Fetal Interaction SLIMP. Mar del Plata, April 2015.
2016-2017. Member of the Organizing Committee VII Congress Latin American Society of Maternal-Fetal Interaction SLIMP. Puerto Varas 2017.
2016-2018. Member of the Board of Directors of the Chilean Society of Reproduction and Development. Director for Santiago.

ADMINISTRATION IN SCIENCE AND RESEARCH MANAGEMENT

2013. Member of the Ethics and Welfare Committee of Laboratory Animals of the School of Medicine, Pontificia Universidad Católica de Chile.
2014. Chairman of the Ethics and Welfare Committee of Laboratory Animals of the School of Medicine, Pontificia Universidad Católica de Chile.
2014-present. Member of the Ethical Scientific Committee on Care and Use of Animals and Research Safety, Pontificia Universidad Católica de Chile.
2018- present. Vice-president Ethical Scientific Committee on Care and Use of Animals and Research Safety, Pontificia Universidad Católica de Chile.
2014-present. Member National Accreditation Commission (CNA) for Postgraduate programs - Chile
2015-present. Member of the Study Group in Biomedicine, National Fund for Scientific and Technological Development (FONDECYT), National Reseach Council.

PARTICIPATION IN SCIENTIFIC SOCIETIES

1998-present. Argentine Society of Biology
2007-present. Chilean Society of Reproduction and Development
2012-present. International Society for Developmental Origins of Health and Disease (DOHaD)
2015- present. Society for the Study of Reproduction.

PARTICIPATION AS REVIEWER

International journals
2009- present. Biological Research
2012- present. Reproduction Fertility and Development
2013-present. Reproduction.
2014- present. PLOS One, PLOS Genetics, Journal of Physiology and Biochemistry, Revista Médica de Chile, Biomedical Research, Biology of Reproduction.
2019- present. Diabetologia.

Funding Agencies
2011- present. National Council for Scientific and Technical Research (CONICET), Argentina.
2012- present. National Fund for Scientific and Technological Development (FONDECYT), National Reseach Council.
2015-present. Fund for the Promotion of Scientific and Technological Development (FONDEF)



Tipos de Producción
 

Article (39)

Attenuation of atherogenic apo B-48-dependent hyperlipidemia and high density lipoprotein remodeling induced by vitamin C and E combination and their beneficial effect on lethal ischemic heart disease in mice
High density lipoprotein cholesterol and proteome in SR-B1 KO mice: lost in precipitation
INTAKE OF RED WINE GRAPE POMACE DECREASED ATHEROSCLEROSIS, ATTENUATED MYOCARDIAL DAMAGE AND INCREASED SURVIVAL IN A MURINE MODEL OF LETHAL CORONARY HEART DISEASE
Transcriptional profiling of embryos lacking the lipoprotein receptor SR-B1 reveals a regulatory circuit governing a neurodevelopmental or metabolic decision during neural tube closure
Deficient Vitamin E Uptake During Development Impairs Neural Tube Closure in Mice Lacking Lipoprotein Receptor SR-BI
Gugulipid causes hypercholesterolemia leading to endothelial dysfunction, increased atherosclerosis, and premature death by ischemic heart disease in male mice
Prolonged Activation of the Htr2b Serotonin Receptor Impairs Glucose Stimulated Insulin Secretion and Mitochondrial Function in MIN6 Cells
Serotonin- and Dopamine-Related Gene Expression in db/db Mice Islets and in MIN6 ? -Cells Treated with Palmitate and Oleate
ANTIOXIDANT DYSFUNCTIONALITY OF HIGH-DENSITY LIPOPROTEINS (HDL) IN DECOMPENSATED DIABETIC PATIENTS
Preventive Letter: Doubling the Return Rate After Gestational Diabetes Mellitus
Early Onset Intrauterine Growth Restriction in a Mouse Model of Gestational Hypercholesterolemia and Atherosclerosis
Excess cholesterol induces mouse egg activation and may cause female infertility
Human fertilization: Epididymal hCRISP1 mediates sperm-zona pellucida binding through its interaction with ZP3
Maternal Hypertriglyceridemia: A Link Between Maternal Overweight-Obesity and Macrosomia in Gestational Diabetes
Physiological and pathological implications of cholesterol
Prenatal nicotine exposure enhances Cx43 and Panx1 unopposed channel activity in brain cells of adult offspring mice fed a high-fat/cholesterol diet
Protective role of high density lipoproteins in sepsis: basic issues and clinical implications
Spermatozoa from mice deficient in Niemann-Pick disease type C2 (NPC2) protein have defective cholesterol content and reduced in vitro fertilising ability
Developmental abnormalities in mouse embryos lacking the HDL receptor SR-BI
Fertilization Induces a Transient Exposure of Phosphatidylserine in Mouse Eggs
Evaluation of Testicular Sperm CRISP2 as a Potential Target for Contraception.
Apolipoprotein A-I deficiency does not affect biliary lipid secretion and gallstone formation in mice
Female infertility due to anovulation and defective steroidogenesis in NPC2 deficient mice
Life-giving caspases: revealing new roles during mouse embryo preimplantation development
Immunocontraceptive properties of recombinant protein DE: implications for the development of novel contraceptives
Participation of cysteine-rich secretory proteins (CRISP) in mammalian sperm-egg interaction
A novel function for CRISP1 in rodent fertilization: Involvement in sperm-zona pellucida interaction
Evidence for the involvement of testicular protein CRISP2 in mouse sperm-egg fusion
Participation of epididymal cysteine-rich secretory proteins in sperm-egg fusion and their potential use for male fertility regulation.
Sperm protein DE mediates gamete fusion through an evolutionarily conserved site of the CRISP family
Expression and structure-function analysis of de, a sperm cysteine-rich secretory protein that mediates gamete fusion.
Molecular mechanisms involved in mammalian fusion
Relationship between the association of rat epididymal protein “DE” with spermatozoa and the behavior and function of the protein
Bicarbonate is required for migration of sperm epididymal protein DE/CRISP-1 to the equatorial segment and expression of rat sperm fusion ability.
Evidence that human epididymal protein ARP plays a role in gamete fusion through complementary sites on the surface of the human egg
Human testicular protein TPX1/CRISP-2: localization in spermatozoa, fate after capacitation and relevance for gamete interaction
Molecular mechanisms involved in gamete interaction.
Morphologic and functional determinants of primordial and primary follicles in the monkey ovary.
Studies on the participation of epididymal sperm protein DE/CRISP-1 in egg activation.

Grant (10)

Cholesterol is bad for eggs as much as eggs are bad for cholesterol.
Magnetic resonance imaging technology for aging related diseases: brain, heart and vessels
Role of Good Cholesterol HDL in Female Fertility

Review (4)

Advances in the physiological and pathological implications of cholesterol
La hipótesis de Pedersen no es suficiente: Otros nutrientes además de la glucosa explicarían la macrosomía fetal en pacientes diabéticas gestacionales con sobrepeso y buen control glicémico
Nutrients other than glucose might explain fetal overgrowth in gestational diabetic pregnancies
Mechanisms regulating hepatic SR-BI expression and their impact on HDL metabolism

Journal Article (3)

Efecto del ciprofibrato sobre el metabolismo del colesterol HDL y la capacidad antioxidante plasmática en el ratón
Papel protector de las lipoproteínas de alta densidad en sepsis: aspectos básicos e implicancias clínicas
Perfil lipídico en mujeres embarazadas sanas de tres regiones de Chile

Editorial Material (1)

Blood lipids during pregnancy: A progressively appreciated subject in basic and clinical research
59
Dolores Busso

Associate Professor

Depto. de Nutrición, Diabetes y Metabolismo

Pontificia Universidad Católica de Chile. Escuela de Medicina

Santiago, Chile

19
Attilio Rigotti

Full Professor

Nutrition, Diabetes and Metabolism

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE - ESCUELA DE MEDICINA - DEPTO DE GASTROENTEROLOGÍA

Santiago, Chile

6
Jose Santos

Full Professor

Department of Nutrition, Diabetes and Metabolism

PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

Santiago, Chile

4
Attilio Rigotti

Full Professor

Nutrition, Diabetes and Metabolism

Pontificia Universidad Católica de Chile

Santiago, Chile

3
Silvana Zanlungo

Full Professor

Gastroenterology

Pontificia Universidad Católica de Chile

Santiago, Chile

3
Loni Berkowitz

Investigador Postdoctoral

Pontificia Universidad Católica de Chile

SANTIAGO, Chile

3
Andrea Leiva

Assistant Professor

Obstetric and Gynecology

PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

Santiago, Chile

2
Pablo Irarrazaval

Professor

Electrical Engineering

Pontificia Universidad Católica de Chile

Santiago, Chile

2
Victor Cortés

Associate professor

Nutrition, Diabetes and Metabolism

Pontificia Universidad Católica de Chile

Santiago, Chile

2
Ricardo Moreno

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE, FACULTAD DE CIENCIAS BIOLÓGICAS, DEPTO. DE FISIOLOGÍA

Santiago, Chile

2
Jose Galgani

ASSOCIATE PROFESSOR

NUTRITION, DIABETES AND METABOLISM; AND UDA-CIENCIAS DE LA SALUD, NUTRICION Y DIETETICA

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE

Santiago, Chile

2
Barbara Samith

Profesor Instructor Adjunto

Ciencias de la Salud

Pontificia Universidad Católica de Chile

Santiago, Chile

2
Juan Orellana

Assistant Professor

Departamento de Neurología

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE

Santiago, Chile

1
Jaime Eugenin

Full Professor

Biology

Universidad de Santiago de Chile

Santiago, Chile

1
Roberto Bravo

Investigador Postdoctoral

Instituto de Nutrición y Tecnología de los Alimentos (INTA), Universidad de Chile

Santiago, Chile

1
Francisca Salas

Investigador Postdoctoral

Nutrición, Diabetes y Metabolismo

Pontificia Universidad Católica de Chile

Santiago, Chile

1
Rommy Von Bernhardi

Full Professor

Neurology

DEPT. NEUROLOGY, FAC. MEDICINE PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE

Santiago, Chile

1
Hugo Verdejo

Profesor Asistente

División de Enferemedades Cardiovasculares

Pontificia Universidad Católica de Chile

Santiago, Chile

1
Valentina Serrano

PROFESOR ASISTENTE

NUTRICION, DIABETES Y METABOLISMO

PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

SANTIAGO, Chile

1
Claudio Vera

Assitant Professor

Obstetrics and Gynecology

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE

Santiago, Chile

1
Alberto Martin

Assistant professor

Centro de Genómica y Bioinformática

Universidad Mayor

Santiago, Chile

1
Alejandro Godoy

Associate Professor

Physiological Sciences

Pontificia Universidad Católica de Chile

Santiago, Chile

1
Tomas Perez-Acle

Full Professor

Computational Biology Lab

Fundación Ciencia para la Vida

Santiago, Chile

1
Guadalupe Echeverria

Manager

Center for Molecular Nutrition and Chronic Diseases

Pontificia Universidad Católica de Chile

Santiago, Chile

1
Eduardo Abbott

Profesor Asistente

Medicina Interna

Pontificia Universidad Católica de Chile

Santiago, Chile

1
Paola Llanos

Profesor Asistente

Instituto de Investigacion en Ciencias Biomédicas

FACULTAD DE ODONTOLOGIA. UNIVERSIDAD DE CHILE

Santiago, Chile

1
Alvaro Martinez

Director Asociado de Propiedad Intelectual y Transferencia Tecnológica

Fundación Ciencia para la Vida

Santiago, Chile