Man

Herman Silva

Professor

Universidad de Chile

Santiago, Chile

Líneas de Investigación


Plant Functional Genomics in fruits and crops to unveil metabolic and signal transduction pathways.

Educación

  •  Licenciate in Biochemistry, UNIVERSIDAD DE CONCEPCION. Chile, 1987
  •  Biochemist, UNIVERSIDAD DE CONCEPCION. Chile, 1988
  •  Ph. D., Plant Biology, Rutgers, The State University of New Jersey. Estados Unidos, 1998

Experiencia Académica

  •   Professor Full Time

    UNIVERSIDAD DE CHILE

    Facultad de Ciencias Agronomicas

    Santiago, Chile

    Noviembre 2015 - A la fecha

Experiencia Profesional

  •   Associate Professor Full Time

    Universidad Andres Bello

    Santiago, Chile

    Enero 2005 - Enero 2010

  •   Assistant Professor Full Time

    Universidad de Chile

    Santiago, Chile

    Enero 1999 - Enero 2005

  •   Ph. D. Student Full Time

    Rutgers University

    New Brunswick, New Jersey, Estados Unidos

    Enero 1994 - Enero 1998

  •   Senior Lab Technician Full Time

    Rutgers University

    Piscataway, New Jersey, Chile

    Enero 1991 - Enero 1993

  •   Instructor Part Time

    Universidad de Chile

    La Pintana, Santiago, Chile

    Enero 1990 - Enero 1991

  •   Associate Professor Full Time

    Universidad de Chile

    Santiago, Chile

    Enero 2011 - Enero 2015

Formación de Capital Humano


Post-Doctoral supervision
• Dra. Karina Ruiz, Project Fondecyt postdoc N°3130624 “How does quinoa deal with salt stress? Evaluation of architectural traits and transcription factors related to stress tolerance genes that make it a successful crop in a challenging environment. October 2012-September 2014 (tutor).
• Dra. Lorena Marchant, Identification of metabolic pathways involved in the aroma of Fragaria chiloensis. April 2007-August 2007.
Doctoral students
• Denisse Zamorano, Doctorate in Agricultural and Veterinary Sciences, Universidad de Chile, ongoing (2017).
• Monica Diaz, Doctorate in Biotechnology, Pontificia Universidad Catolica de Valparaiso y Universidad Federico Snata Maria, ongoing (2017).
• Daniela Arias, Doctorate in Biotechnology, Universidad de Chile, ongoing (2017).
• Loreto Prat, Doctorate in Biotechnology, Universidad Andres Bello, finished 12/2014.
• Sergio Diez de Medina, Doctorate in Biotechnology, Universidad Andres Bello, finished 05/2014.
• Juan Carlos Rios, Doctorate in Biotechnology, Universidad Andres Bello, finished 05/2014.
• Lorena Marchant, Doctorate in Biology, Facultad de Ciencias, Universidad de Chile. Co-director of thesis. Finished: 2004 (Conicyt and Andes Fundation fellowship).
Master students
• Javiera Fuentes, MSc. in Agricultural Sciences, Universidad de Chile, ongoing (2017).
• Barbara Valenzuela, MSc. in Agricultural Sciences, Universidad de Chile, ongoing (2017).
• Jorge Moraga, MSc. in Agricultural Sciences, Universidad de Chile, finished 12/2016.
Undergraduate students
• Rodrigo Corral, Agronomist Engineering, Universidad de Chile, Undergraduate Thesis, ongoing (2017).
• Gaëlle Calvez, Agronomist Engineering, Universidad de Chile, Undergraduate Thesis, ongoing (2017).
• Natalia Molina, Agronomist Engineering, Universidad de Chile, Undergraduate Thesis, 2017.
• Francisca Matus, Agronomist Engineering, Universidad de Chile, Undergraduate Thesis, 2013.
• María Soledad Cabrera, Molecular Biotechnology Engineering, Faculty of Sciences, Universidad de Chile, Undergraduate Thesis, 2011.
• Gabriel Ballesteros, Bioinformatics Engineering, Universidad de Talca, Undergraduate Thesis, 2010.
• Alejandra Cifuentes, Biochemistry, Faculty of Biological Sciences, Pontificia Universidad Católica, Undergraduate Thesis, 2010.
• Jonathan Maldonado, Molecular Biotechnology Engineering, Faculty of Sciences, Universidad de Chile, Undergraduate Thesis, 2010.
• Margarita Santiago, Molecular Biotechnology Engineering, Faculty of Sciences, Universidad de Chile, Undergraduate Thesis, 2007.
• Juan Saba, Civil Engineering in Computer Sciences, Facultad de Ciencias Físicas y Matemáticas, Universidad de Chile. Undergraduate Thesis, 2006 (co-adviser).
• Francisco Duarte, Biochemistry, Universidad de Santiago. Undergraduate Thesis, 2005.
• Andres Tittarelli, Molecular Biotechnology Engineering, Faculty of Sciences, Universidad de Chile, Undergraduate Thesis, 2005.
• Nicolas Loira, Civil Engineering in Computer Sciences, Facultad de Ciencias Físicas y Matemáticas, Universidad de Chile. Undergraduate Thesis, 2005 (co-adviser).
• Luis Milla, Molecular Biotechnology Engineering, Faculty of Sciences, Universidad de Chile, Undergraduate Thesis, 2003.

TEACHING RESPONSABILITIES
Currently: Biochemistry for agronomist undergraduate students (Universidad de Chile) and Advanced Genetics for Ph.D. students (Agricultural and Veterinary Sciences Program).


Difusión y Transferencia


2015-2017: Rescue and valorization of the blod-flesh peach by generating a bank of live germplasm and the study of its nutritional and culinary properties in the town of Constitucion, Maule Region.(FIA; PYT-2015-0390). This project allowed the establishment of an association for family farmers to comercialize their products and get new peach plants with higher concentration of antioxidants.


Premios y Distinciones

  •   Academic Forum Chile - Japan 2014

    CONICYT

    Chile, 2014

    Member of the chilean delegation to participate in the group Biological Sciences: Genes, cell and organisms. Chilean coordinator: Dr. Miguel Allende; Japanese coordinator: Dr. Hiroyuki Takeda

  •   Fellowship to attend to RGC7 (7th International Rosaceae Genomics Conference). Seattle, WA

    RosBreed

    Estados Unidos, 2014

    7th International Rosaceae Genomics Conference.


Tipos de Producción
 

Conference Poster (48)

Effect of gibberellic acid on cracking, maturity and fruit quality of sweet cherry trees.
Functional characterization of the transcriptional co-factor Par1 by over-expression in carrot.
Present and future of marker-assisted breeding in sweet and sour cherry.
The Sweet Cherry Genome: A New Sequencing Project.
Transcriptome Characterization of V. pubescens and V. chilensis: Two Phenotipically Contrasting Papaya Species.
Transcriptomic Analysis of ‘Garnem’ Rootstock By RNA-SEQ Reveals Genes and GENE Ontologies Involved in Drought Response.
Bioinformatics and Molecular Analysis of Factors Involved in Developmental and Cracking Tolerance in Different Varieties of Sweet Cherry.
Genetic structure based on EST-SSR: A promising tool for fruit color selection in Japanese plum (Prunus salicina L.) breeding programs.
Correlation Between Alkanes and Cracking in Different Sweet Cherries Varieties-Sweet Cherry.
De novo transcriptome analysis of Daucus carota modified root under dark or light grown conditions.
Development of a saturated linkage map in Prunus salicina L. using genotyping by sequencing (GBS).
Effect of gibberelic acid on cracking and gene expression in sweet cherry fruits.
Genetic straucture based on EST-SSR: A promising tool for fruit color selection in Japanese plum (Prunus salicilina L.) breeding programs.
Genomic studies on a highly complex trait in sweet cherry: tolerance to rain-induced fruit cracking.
High-density SNP-based genetic map of Japanese plum (Prunus salicina L.).
Molecular phylogeny of Mutisia (Asteraceae, Mutisieae) with biogeographic implications in the Neotropical disjunction.
RNA seq as a tool to study the effect of handling stress in the expression of genes associated with the immune response of red cusk-eel (Genypterus chilensis).
The Peach v2.0 Release: An Improved Genome Sequence for Bridging the Gap Between Genomics and Breeding in Prunus.
Composición aromática de Fragaria chiloensis (L.) Mill y Fragaria x ananassa Duch.
Correlation between the amount of alkanes and cracking in different sweet cherries varieties.
Factores moleculares implicados en la partidura del fruto en cerezo.
Genetic and morphological analysis of the endangered austral papaya (Vasconcellea chilensis (Planch. ex A. DC.) Solms).
Respuestas al estrés salino en cultivares chilenos de quinua: caracterización “In Vitro”.
SNPs, SSR, ISSR and phenotypic evaluation of Japanese plum (Prunus salicina L.) cultivars.
Analysis of differentially expressed genes from cherry fruit (Prunus avium) among cultivars with contrasting levels of cracking tolerance.
Approaches Enabling Molecular Breeding of Sweet Cherry.
Construction of a highly dense map of sweet cherry and its comparative analyses with others Prunus linkage maps.
Development of EST SSR based on the first transcriptome of Prunus salicina.
Genetic diversity and paternity test in Japanese plum (Prunus salicina LINDL).
Genotypic and phenotypic evaluation of Japanese plum (Prunus salicina L.) cultivars.
Respuestas al estres salino en genotipos de quinoa originarios de Chile: desde su arquitectura hasta sus genes.
The cytokinin response pathway during Prunus persica fruit development.
Transcriptional profile of sweet cherry fruits with different tolerance to cracking.
Chemical characterization of Fragaria chiloensis aroma: Gas chromatography-olfactometry and sensory reconstitution study.
Comparative genomic analysis in Prunoideae.
Construction and comparative analyses of highly dense linkage maps of two sweet cherry intra-specific progenies of commercial cultivars.
Correlation between cracking, CER6 gene expression and alkane concentration in sweet cherry fruits.
Isolation of genes differentially expressed during refrigerated storage involved in internal breakdown of Japanese plum (Prunus salicina Lindl.) fruits.
RNA seq as a tool to unveil the transcriptome of different sweet cherry varieties.
RNA-seq expression analyses in sweet cherries to try to understand cracking tolerance.
RNA-seq expression analyses in sweet cherries with differential susceptibility to cracking.

Journal Article (42)

Characterization of the high-affinity phosphate transporter PHT1;4 gene promoter of Arabidopsis thaliana in transgenic wheat
Comparing salt-induced responses at the transcript level in a salares and coastal-lowlands landrace of quinoa ( Chenopodium quinoa Willd)
Genetic, cytological and molecular characterization of chia ( Salvia hispanica L.) provenances
Transcriptional Responses of Chilean Quinoa (Chenopodium quinoa Willd.) Under Water Deficit Conditions Uncovers ABA-Independent Expression Patterns
Transcriptomic analysis of the hepatic response to stress in the red cusk-eel (Genypterus chilensis): Insights into lipid metabolism, oxidative stress and liver steatosis
De novo transcriptome assembly of 'Angeleno' and 'Lamoon' Japanese plum cultivars (Prunus salicina)
Genetic structure based on EST–SSR: a putative tool for fruit color selection in Japanese plum (Prunus salicina L.) breeding programs
Salares versus coastal ecotypes of quinoa: Salinity responses in Chilean landraces from contrasting habitats
Association between the concentration of n-alkanes and tolerance to cracking in commercial varieties of sweet cherry fruits
Characterization of the acetohydroxyacid synthase multigene family in the tetraploide plant Chenopodium quinoa
mRNA-seq reveals skeletal muscle atrophy in response to handling stress in a marine teleost, the red cusk-eel (Genypterus chilensis)
Genetic and morphological characterization of the endangered Austral papaya Vasconcellea chilensis (Planch. ex A. DC.) Solms
Identification of volatile compounds associated with the aroma of white strawberries (Fragaria chiloensis)
Sequencing and de novo assembly of the red cusk-eel (Genypterus chilensis) transcriptome
Characterization of cytokinin signaling and homeostasis gene families in two hardwood tree species: Populus trichocarpa and Prunus persica
Comparative genomics analysis in Prunoideae to identify biologically relevant polymorphisms
Construction and Comparative Analyses of Highly Dense Linkage Maps of Two Sweet Cherry Intra-Specific Progenies of Commercial Cultivars
Profiling carbohydrate composition, biohydrogen capacity, and disease resistance in potato
The high-quality draft genome of peach (Prunus persica) identifies unique patterns of genetic diversity, domestication and genome evolution
A molecular marker approach using intron flanking EST-PCR to map candidate genes in peach (Prunus persica)
The genome of woodland strawberry (Fragaria vesca)
Comparative EST transcript profiling of peach fruits under different post-harvest conditions reveals candidate genes associated with peach fruit quality
Isolation and functional characterization of cold-regulated promoters, by digitally identifying peach fruit cold-induced genes from a large EST dataset
Identification of woolliness response genes in peach fruit after post-harvest treatments
Isolation and comparative analysis of the wheat TaPT2 promoter: identification in silico of new putative regulatory motifs conserved between monocots and dicots
Arabidopsis thaliana: A model host plant to study plant-pathogen interaction using Chilean field isolates of Botrytis cinerea
AtUTr1, a UDP-glucose/UDP-galactose transporter from Arabidopsis thaliana, is located in the endoplasmic reticulum and up-regulated by the unfolded protein response
Seasonal variation in the development of chilling injury in 'O'Henry' peaches
The Ca2+ pump inhibitor, thapsigargin, inhibits root gravitropism in Arabidopsis thaliana
A Rapid and Efficient Method for Purifying High Quality Total RNA from Peaches (Prunus persica) for Functional Genomics Analyses
Phosphate deficiency regulates phosphoenolpyruvate carboxylase expression in proteoid root clusters of white lupin
Transport of UDP-galactose in plants - Identification and functional characterization of AtUTr1, an Arabidopsis thaliana UDP-galactose/UDP-glucose transporter
Nitric oxide and salicylic acid signaling in plant defense
NPR1 differentially interacts with members of the TGA/OBF family of transcription factors that bind an element of the PR-1 gene required for induction by salicylic acid
Characterization of a new Arabidopsis mutant exhibiting enhanced disease resistance
Engineering disease and pest resistance in plants
Protein dephosphorylation mediates salicylic acid-induced expression of PR-1 genes in tobacco
Induction, modification, and transduction of the salicylic acid signal in plant defense responses
Active oxygen species in the induction of plant systemic acquired resistance by salicylic acid
Aislamiento, selección y caracterización de hongos ligninolíticos chilenos (Isolation, Selection and Characterization of chilean ligninolytic fungi)
Characterization of Palo Podrido, a Natural Process of Delignification in Wood
A MECHANISM OF ACTION OF SALICYLIC-ACID IN PLANT-DISEASE RESISTANCE

Grant (22)

Creación de una unidad de microscopia avanzada en el Campus Sur
Study of plant cell polarity, The Millennium Nucleus in Plant Cell Biology (PCB)

Conference Paper (11)

Functional genomics as a tool for improving fruit quality.

Review (4)

Breeding in peach, cherry and plum: from a tissue culture, genetic, transcriptomic and genomic perspective
Cracking in sweet cherries: A comprehensive review from a physiological, molecular, and genomic perspective
Proteomic analysis of peach fruit mesocarp softening and chilling injury using difference gel electrophoresis (DIGE)
JUICE: a data management system that facilitates the analysis of large volumes of information in an EST project workflow

Book Section (2)

Functional Genomics: Transcriptomics
Innovación, Desarrollo y Negocios en Biotecnología

Abstract (1)

Proceedings Paper (1)

Identification of SSRs with Polymorphisms between Different Prunus avium Cultivars
136
Herman Silva

Professor

Departamento de Produccion Agricola

Universidad de Chile

Santiago, Chile

19
Lee Meisel

Associate Professor

Institute of Nutrition and Food Technology (INTA), University of Chile

Macul, Chile

9
Jonathan Maldonado

Postdoc

Instituto de Nutrición y Tecnología de los Alimentos

Universidad de Chile

Santiago, Chile

8
Liliana Cambiazo

Associate Professor

Unidad de Nutrición Básica

UNIVERSIDAD DE CHILE - INTA

Santiago, Chile

6
Basilio Carrasco

ASSOCIATE PROFESSOR

CIENCIAS VEGETALES

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE

SANTIAGO, Chile

5
Mauricio González

Associate Professor

UNIVERSIDAD DE CHILE - INSTITUTO DE NUTRICION Y TECNOLOGIA DE ALIMENTOS

Santiago, Chile

4
Andrea Morales

Académico de planta, encargada de Investigación

Salud

Universidad Santo Tomas

La Serena, Chile

3
Juan Valdés

Head lab

Ciencias Biologicas

UNIVERSIDAD ANDRÉS BELLO

Santiago, Chile

3
Lorena Norambuena

Associate Proffesor

Biology

UNIVERSIDAD DE CHILE

Santiago, Chile

3
Andrés Tittarelli

Investigador Asociado

Fraunhofer Chile Research

Santiago, Chile

2
Alfredo Molina

UNIVERSIDAD ANDRES BELLO

SANTIAGO, Chile

2
Claudio Meneses

Associate Professor

Ciencias Biológicas

UNAB

Santiago, Chile

2
Juan Manuel Estrada

Director

Facultad de Ecología y Recursos Naturales

Universidad Andrés Bello

Quintay , Chile

2
PAULA VIZOSO

Researcher

Universidad Mayor

SANTIAGO, Chile

2
Juan Rios

Postdoctorante

Biología

Universidad Andres Bello

Santiago, Chile

2
Sergio Diez-de-Medina

Director técnico Proyecto FIC 2015

Vicerrectoría de Investigación, Innovación y Poistgrado

Universidad Arturo Prat

Iquique, Chile

2
Gaston Muñoz

assistant professor

Ciencias Biologicas

Universidad San Sebastian

Valdivia, Chile

2
Eduardo Gratacos

Director

Centro Ceres

Centro Ceres

Quillota, Chile

1
Raúl Herrera

Académico

Biología Molecular y Biotecnología

Universidad de Talca

Talca, Chile

1
Cristian Gallardo

Profesor Asociado

Departamento de Oceanografía

Universidad de Concepción

Concepcion, Chile

1
Reinaldo Campos

Titular Professor - Director

Facultad de Ciencias Biológicas

Universidad Nacional Andrés Bello

Santiago, Chile

1
Claudio Meneses

UNIVERSIDAD ANDRÉS BELLO

Santiago, Chile

1
Eduardo Agosin

Full Professor

Chemical and Bioprocess Engineering

PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

Santiago, Chile

1
Karina Ruiz

Investigador

Agricultura del Desierto

Universidad Arturo Prat

Iquique, Chile

1
Carolina Torres

Professor/Researcher

Facultad de Ciencias Agrarias

UNIVERSIDAD DE TALCA

Talca, Chile

1
Gerardo Tapia

Encargado Recursos Geneticos Vegetales

INSTITUTO DE INVESTIGACIONES AGROPECUARIAS, INIA

Chillán, Chile

1
Carole Diaz

Coordinadora de Desarrollos Tecnológicos

Universidad Santo Tomás

Santiago, Chile

1
Luis Milla

Profesor Asistente

Escuela de Medicina

Universidad de Santiago

Santiago, Chile

1
Gustavo Lobos

Assistant Professor

Horticulture

FACULTDAD DE CIENCIAS AGRARIAS, UNIVERSIDAD DE TALCA

Talca, Chile