Man

Juan Carlos Saez Carreño

Professor

Pontificia Universidad Católica de Chile

Santiago, Chile

Líneas de Investigación


Regulation and function of gap junctions and hemichannels formed by connexins or pannexins in cells of the brain and skeletal muscles under normal and pathological conditions.

Educación

  •  Neuroscience, ALBERT EINSTEIN COLLEGE OF MEDICINE. Estados Unidos, 1986
  •  Biochemist, UNIVERSIDAD DE CONCEPCION. Chile, 1979

Experiencia Académica

  •   Instructor Full Time

    UNIVERSIDAD DE CONCEPCION

    Biological Sciences

    Concepcion, Chile

    Enero 1979 - Enero 1983

  •   Assistant Professor Full Time

    ALBERT EINSTEIN COLLEGE OF MEDICINE

    New York, Estados Unidos

    Enero 1987 - Enero 1993

  •   Assistant Professor Full Time

    PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

    Biological Sciences

    Santiago, Chile

    Enero 1993 - Enero 1994

  •   Associate Professor Full Time

    Biological Sciences

    Santiago, Chile

    Enero 1995 - Enero 2002

  •   Full Professor Full Time

    Biological Sciences

    Santiago, Chile

    Enero 2003 - A la fecha

Experiencia Profesional

  •   INstructor Full Time

    Universidad de Concepcion

    Concepcion, Chile

    Enero 1979 - Enero 1983

  •   Assistant Professor Full Time

    Albert Instein College of Medicine

    New York, Estados Unidos

    Enero 1987 - Enero 1993

  •   Assistant Professor Full Time

    Pontificia Universidad Católica de Chile

    Santiago, Chile

    Enero 1993 - Enero 1994

  •   Associate Professor Full Time

    Pontificia Universidad Católica de Chile

    Santiago, Chile

    Enero 1994 - Enero 2002

  •   Professor Full Time

    Pontificia Universidad Católica de Chile

    Santiago, Chile

    Enero 2003 - A la fecha

Formación de Capital Humano


3 postoral fellows

-Carlos Puebla. FONDECYT. (3130662). "Regulation glucose transport through its specific transporters and hemichannels by essential fatty acids in the small intestine".
-Adam Aguirre. FONDECYT. (3130632)."Involvement of hemichannels in glia pre-activation by auditory stress to inflammation promoted by poly (I:C) and consequences on neuronal arborization and survival".
-Jose Luis Vega. FONDECYT.(3120006). “Regulation of connexin-43 hemichannels properties by PKC, PKA and MAP kinases”.

32 graduate students and 6 undergraduate students

- 2011-present. Bruno Cisterna.Doctor Ciencias Fisiológicas, PUC. "Role of connexin hemichannels in muscle atrophy induced by denervation".
- 2012-present. Elsa Fritz,(Co-mentor with Dr. Jorge Campusano) Doctor Ciencias Fisiológicas, PUC. "Role of innexins on the coordinated circadian of behavior in Drosophila melanogaster".
- 2013-present. Gonzalo Gomez, Doctor Ciencias Fisiológicas, PUC."The R hoA/ROCK pathway regulates the connexin43 hemichannel and gap junctional channel activity in angiotensin-induce kidney injury.
- 2013-present. Dusan Recordon. (co-mentor with Dr. Gareth Owen). Doctor Ciencias Fisiológicas, PUC. "Purinergic signaling in the vasculogenesis-like process".
- 2009-present. Carola Marturana, Doctor Ciencias Fisiológicas, PUC. "Involvement of pannexin1 hemichannels of oligodendrocytes in amyelinization during prenatal stress".
- 20111-present Anibal Vargas, Doctor Ciencias Fisiológicas, PUC. "Involvement of connexin hemichanels in muscle atrophy induced by cachexia".
- 2009-present. Paloma Hacha, Doctor Ciencias Fisiológicas, PUC. Involvement of hemichannels in the degranulation response induced by immunoglobulin E in murine mast cells”
- 2012-2014. Carolina Urrutia, (co-mertor with DR. Victoria Velarde). Master in Science: Free Radicals and Biomedicine”. University of Vaparaíso. "Activation of hemichannels formed by connexins promotes Ca2+ influx, oxidatve stress and cell death, which is prevented by antioxidant agents that block hemichannles”.
- 2009-2013. Maximiliano Rovegno. (Co-mentor with Dra. Rommy von Benhardi). Doctorado en Ciencias Médicas, PUC. “Activation of inflammation in astrocytes induced by ATP release via connexin hemichannels in an in vitro model of brain trauma".
- 2009-2013. Raúl Lagos Cabré. (Co-mentor with Dr. Ricardo Moreno). Doctorado en Ciencias Fisiológicas, PUCC. “Modulation of the ADAM protein family by xenostrogens induces apoptosis in male germinal cells”.
- 2009-presente. Daniela Salas. (Co-mentor with Dr. Sergio Lavandero). Doctorado en Bioquímica, Universidad de Chile. “Regulation of Cx43 hemichannels by metabolic stress”.
- 2008-2012. Pablo J. Sáez. Candidato a Doctor Ciencias Fisiológicas, PUC. Regulation and funtion of hemichannels and gap junction channels at the interaction between dendritic cell and T lymphocytes.
- 2009-2012. Vania Figueroa. (Co-mentor with Dr. Agustín Martínez). Doctorado en Neurociencias, Universidad de Valparaíso. Activation of hemichannels formed by hCx26 and hPanx1 under physiologic and pathologic conditions and its possible role in the etiopatognesis of defness.
- 2008-2012. Luis A. Cea. Doctor Ciencias Fisiológicas, PUC. The activity controls the levels of connexins 39, 43 and 45 in rat skeletal muscles.
- 2008-2011. Kenji Shoji. Doctor Ciencias Fisiológicas, PUC. T-lymphocytes present Panx1 channels coupled to P2X7 receptor that mediate ATP-induced cell death induced by extracellular ATP.
- 2008-presente. Romina Hernández (Co-tutor con Dra. M.V. Velarde). Candidato a Doctor Ciencias Fisiologicas, PUC. Effect of boldine in diabetic nephropaty in rats.
- 2008-2010. Juan A. Orellana. Doctor en Ciencias Biológicas, mención Ciencias Fisiológicas, PUC. Role and regulation of astroglial channels formed by connexin43 in neuronal death induced by hypoxia in high glucose: potentiation by neurodegenerative agents.
- 2005-2010. Manuel A. Riquelme. Doctor en Ciencias Biológicas, mención Ciencias Fisiológicas, PUC. Role of panneixn1 hemichannels and extracellular ATP in myogenic commitment and potentiation of muscle contraction in adult muscles.
- 2003-2008. Carolina Gatica de la Puente. Doctor en Ciencias Biológicas, mención Ciencias Fisiológicas, PUC. Role of hemichannels and gap junction channels in neuronal differentiation and myogenic commitment acquisition in cell lines.
- 2005-2008. Helmuth Sánchez. Doctor en Ciencias Biológicas, mención Ciencias Fisiológicas, PUC. Involvement of connexin32 based channels in cell responses of two in vitro models of ischemia-reperfusion.
- 2007-2008. Kurt A. Schalper. Doctor en Ciencias Médicas, PUC. Modulation of connexin hemichannel functional state by different physicochemical stimuli.
- 2003-2005. Mauricio Retamal. Doctor en Ciencias Biológicas, mención Ciencias Fisiológicas. PUC. Hemichannels formed by connexin43 are sensitive to redox changes.
- 2003-2005. Mauricio Garré. (Co-Director con la Dr. Veronica Abudara). Maestría en Biología, opción Neurociencias. PEDECIBA, Uruguay.Effects of fibroblast growth factor FGF-1 on the intercellular communication medoated by gap junction channels on the neurotoxic phenotype of spinal astrocytes
- 2001-2005. (Co-Director con el Dr. M. Boric) Loreto P. Véliz. Doctor en Ciencias Biológicas, mención Ciencias Fisiológicas, PUC. Expression and function of gap junctions between leukocytes and endothelial cells during inflammation.
- 1999-2003. Sra. Liliana Corvalán. Doctor en Ciencias Biológicas, mención Ciencias Fisiológicas, PUC. “Gap junctions in contacts dendrict cell-T cell contacts”.
- 1999-2003. Dr. Jorge Contreras. Doctor en Ciencias Biológicas, mención Ciencias Fisiológicas, PUC. “Biophysical properties and mechanism of action of hemichannels formed by connexin43: possible participation in cell death induced by ischemia”.
- 1999-2004. Roberto Araya. Doctor en Ciencias Biológicas, mención Ciencias Fisiológicas, PUC. Papel de los receptores purinérgicos y de las uniones en hendidura en el proceso de diferenciación del músculo esquelético.
- 1998-2001. Dr. Eliseo A. Eugenín. Doctor en Ciencias Biológicas, mención Ciencias Fisiológicas, PUC. “Uniones en hendidura (UH) entre macrófagos: identificación de conexinas, regulación de su expresión y su función en procesos metabólicos y celulares”.
- 1997-2000. Dr. María C. Brañes. Doctor en Ciencias Biológicas, mención, Biología Celular y Molecular. PUC. “Polymorphonuclear cells and endothelial cells form homo end heterocellular gap junctions regulated by proinflammatory agents”.
- 1996-1998. Dr. Hernán González. Doctor en Ciencias Médicas. PUC. “Effect of endotoxemia on hepatic gap junctions”.
- 1996-1999. Dr. Agustín Martínez. Doctor en Ciencias Biológicas, mención Ciencias Fisiológicas. PUC. “In vitro regulation of glial gap junctions by inflammatory conditions”.
- 1989-1992. Dr. Viviana M. Berthoud, Department of Neuroscience, Albert Einstein College of Medicine, Nueva York, E.E.U.U. Ph.D. in Sciences. “Regulation of Gap Junctions”.

21 Undergradute thesis

- 2013-2014 Angelica Benvenuto.Biochemist, PUC. "Characterization of hemichannels and gap junction channels formed by connexina43 y panxexin 1 en adipocytes"
- 2011. (Co-mentor with Dr. Xavier Figueroa) Mauricio A. Lillo. Biochemist, PUC. Involvement hemichannels formed by connexins or panneins in NO transporta cross the Palms membrane in the vascular wall.
- 2009. Anibal Vargas. Lic. Biología, PUC. Characterization of antimicin A as inhibitor of hemichannels formed by connexins.
- 2007-2009. (Co-mentor with Dr. Sergio Lavandero). Daniela P. Salas. Biochemist, Universidad de Chile. Hemichannels formed by connexins in volume regulation in myocardiocytes exposed to hyposmotic stress.
- 2009. Paola Soto. Biologist, PUC. Study on channels formed by connexin32 in a cell line derived from oligodendrocytes.
- 2008. Marlene Arismendi, Industrial Chemist, UTEM, Purification and biological activity of compunds derived from Boldo on channels formed by connexins.
- 2008. Diego Hernández, Biologist, PUC. Regulation of astroglial and neuronal channels formed by connexins in neurodegenerative processes.
- 2009. Natalia Vega, Biochemist, PUC. Regulation of cell membrane permeability by phosphorylation of hemichannels formed by connexin43 via protein kinase C (PKC).
- 2008. Paloma Harcha, Biologist, PUC. D2SC1 cells express functional hemichannels formed by pannexina1.
- 2006. Ariel Orellana. Biologist, PUC. Acquisition of myogenic commitment of reserve C2C12 cells requires activation of P2X receptors.
- 2006. Pablo J. Sáez, Biologist, PUC. ATP and TNF-? induce formation gap junction channels in microglia.
- 2006. Kenji Shoji, Biologist, PUC. The redox sensor of hemichannels formed by connexin43 is located in the carboxyl terminus of the protein subunit.
- 2005. Juan Andrés Orellana, Biologist, PUC. Chlorpromazine inhibits the intercellular communication mediated by gap junctions in Gn-11 cells and astrocytes.
- 2005. Constanza J. Cortés, Biologist, PUC. Opening of Cx43 hemichannels in mediated by oxidative
mechanisms in astrocytes”.
- 2005. Nicolás Palacios, Biologist, PUC. Modulation of the functional state of connexin-based channels by sexual hormones and tamoxifen in a cells line derived from human breast cancer.
- 2002-2003. Manuel Riquelme-Biochemist, PUC. P2X receptors and conneixn-based channels are neede for adquisition of myogenic commitment.
- 1998-1999. Patricio Orio-Biochemist, Universidad de Chile. Molecular characterization and function gap junctions Express by Peripherals huma lymphocytes.
- 1997. Alejandro Sepúlveda-Biochemist, Univ. Austral de Chile. Gap junctions between antigen presenting cells and T cells: characterization and regulation of connexins.
- 1997. Eliseo Eugenín-Biochemist, Univ. Austral de Chile. Regulation of glycogen stores by norepinephrine in rat pineal gland: role of gap junctions.
- 1994-1995. Francisco Scheihing- Biochemist, Universidad Católica de Valparaíso. Effect of leptocarpin on gap junctions of epithelial cell lines.
- 1996. Erwin Strahsburger-Biochemist, Univ. Austral de Chile. Regulation of gap junctional Communications in astroglial cells of the pineal gland.


Difusión y Transferencia


3 workshops and three meeting symposia.


Premios y Distinciones

  •   Scopus

    Elsevier Editorial

    Chile, 2008

    Highest productivity and impact among Chilean Scientist in Pharmacology, Biochemistry, Cell Biology and Molecular Biology


Tipos de Producción
 

Dtp Import/Article (85)

Cannabinoids prevent the amyloid β-induced activation of astroglial hemichannels=> A neuroprotective mechanism.
Connexin-Dependent Neuroglial Networking as a New Therapeutic Target.
Connexins and Pannexins in Bone and Skeletal Muscle.
Inhibition of glial hemichannels by boldine treatment reduces neuronal suffering in a murine model of Alzheimer's disease
On Biophysical Properties and Sensitivity to Gap Junction Blockers of Connexin 39 Hemichannels Expressed in HeLa Cells.
Regulation of Connexin-Based Channels by Fatty Acids.
A Soluble Activin Receptor IIB Fails to Prevent Muscle Atrophy in a Mouse Model of Spinal Cord Injury.
Connexin hemichannels explain the ionic imbalance and lead to atrophy in denervated skeletal muscles.
Connexin Hemichannels=> Methods for Dye Uptake and Leakage.
Dexamethasone-induced muscular atrophy is mediated by functional expression of connexin-based hemichannels.
Dexamethasone-induced muscular atrophy is mediated by functional expression of connexin-based hemichannels.
Fast skeletal myofibers of mdx mouse, model of Duchenne muscular dystrophy, express connexin hemichannels that lead to apoptosis.
High glucocorticoid levels during gestation activate the inflammasome in hippocampal oligodendrocytes of the offspring.
Linoleic acid permeabilizes gastric epithelial cells by increasing connexin 43 levels in the cell membrane via a GPR40- and Akt-dependent mechanism.
The absence of dysferlin induces the expression of functional connexin-based hemichannels in human myotubes.
Connexin43 hemichannels mediate secondary cellular damage spread from the trauma zone to distal zones in astrocyte monolayers.
Hemichannels Are Required for Amyloid β-Peptide-Induced Degranulation and Are Activated in Brain Mast Cells of APPswe/PS1dE9 Mice.
Keratitis-Ichthyosis-Deafness Syndrome-Associated Cx26 Mutants Produce Nonfunctional Gap Junctions but Hyperactive Hemichannels When Co-Expressed With Wild Type Cx43.
Neural progenitor cells isolated from the subventricular zone present hemichannel activity and form functional gap junctions with glial cells.
Pannexin channels mediate the acquisition of myogenic commitment in C2C12 reserve cells promoted by P2 receptor activation.
Regulation of pannexin and connexin channels and their functional role in skeletal muscles.
Restraint stress increases hemichannel activity in hippocampal glial cells and neurons.
Role of Akt and Ca2+ on cell permeabilization via connexin43 hemichannels induced by metabolic inhibition.
Characterization of a novel water pocket inside the human Cx26 hemichannel structure.
Extracellular gentamicin reduces the activity of connexin hemichannels and interferes with purinergic Ca(2+) signaling in HeLa cells.
HIV increases the release of dickkopf-1 protein from human astrocytes by a Cx43 hemichannel-dependent mechanism.
Hunting for connexin hemichannels.
Neuronal involvement in muscular atrophy.
Opening of pannexin- and connexin-based channels increases the excitability of nodose ganglion sensory neurons.
Pannexin 1 channels in skeletal muscles.
Pannexin 1 channels: new actors in the regulation of catecholamine release from adrenal chromaffin cells.
Pannexin 1 regulates bidirectional hippocampal synaptic plasticity in adult mice
Pannexin1 channels act downstream of P2X 7 receptors in ATP-induced murine T-cell death.
Regulation of hemichannels and gap junction channels by cytokines in antigen-presenting cells.
ATP is required and advances cytokine-induced gap junction formation in microglia in vitro.
Boldine prevents renal alterations in diabetic rats.
Cerebral ischemic injury is enhanced in a model of oculodentodigital dysplasia.
Connexin and pannexin hemichannels in brain glial cells: properties, pharmacology, and roles.
De novo expression of connexin hemichannels in denervated fast skeletal muscles leads to atrophy.
Diffusion of nitric oxide across cell membranes of the vascular wall requires specific connexin-based channels.
Diffusion of nitric oxide across cell membranes of the vascular wall requires specific connexin-based channels.
Disruption in connexin-based communication is associated with intracellular Ca²⁺ signal alterations in astrocytes from Niemann-Pick type C mice.
FasL-triggered death of Jurkat cells requires caspase 8-induced, ATP-dependent cross-talk between Fas and P2X7 receptors.
Gap junction intercellular communication mediated by connexin43 in astrocytes is essential for their resistance to oxidative stress.
Linoleic acid induces opening of connexin26 hemichannels through a PI3K/Akt/Ca2+-dependent pathway.
Pannexin1 hemichannels are critical for HIV infection of human primary CD4+ T lymphocytes.
Pannexin1 hemichannels are critical for HIV infection of human primary CD4+ T lymphocytes.
Possible involvement of TLRs and hemichannels in stress-induced CNS dysfunction via mastocytes, and glia activation.
Role of gap junctions and hemichannels in parasitic infections.
The extracellular ATP required for potentiation of the adult skeletal muscle contraction is released through pannexin 1 based channels
Connexin 36 is expressed in beta and connexins 26 and 32 in acinar cells at the end of the secondary transition of mouse pancreatic development and increase during fetal and perinatal life.
Connexin and pannexin hemichannels in inflammatory responses of glia and neurons.
Connexin- and pannexin-based channels in normal skeletal muscles and their possible role in muscle atrophy.
Connexin-based channels in astrocytes: how to study their properties.
Connexins 26, 32 and 36 are expressed in a cell-specific manner at the end of the secondary transition of fetal mouse pancreas development and increase during fetal and perinatal life.
Critical role of the first transmembrane domain of Cx26 in regulating oligomerization and function.
Glial connexin expression and function in the context of Alzheimer's disease.
Glial hemichannels and their involvement in aging and neurodegenerative diseases.
Glucose increases intracellular free Ca2+ in tanycytes via ATP released through connexin 43 hemichannels
Modulation of gap junction channels and hemichannels by growth factors.
Permeation of calcium through purified connexin 26 hemichannels.
Regulation of intercellular calcium signaling through calcium interactions with connexin-based channels.
Release of gliotransmitters through astroglial connexin 43 hemichannels is necessary for fear memory consolidation in the basolateral amygdal
Release of gliotransmitters through astroglial connexin 43 hemichannels is necessary for fear memory consolidation in the basolateral amygdala.
The role of gap junction channels during physiologic and pathologic conditions of the human nervous system.
ATP and glutamate hemichannels mediate neuronal death through released via astroglial connexin 43 activation of pannexin 1 hemichannels.
Cation permeation through connexin 43 hemichannels is cooperative, competitive and saturable with parameters depending on the permeant species
FGF -1 induces ATP release from spinal astrocytes in culture and opens pannexin and connexin hemichannels.
Hemichannels in the Neurovascular Unit and White Matter Under Normal and Inflamed Conditions.
Cell membrane permeabilization via connexin-hemichannels in living and dying cells.
Connexin43 is a pH-gated plasma membrane calcium channel.
Hypoxia in high glucose followed by reoxygenation in normal glucose reduces the viability of cortical astrocytes through increased permeability of connexin 43 hemichannels.
Inhibition of Cx43 hemichannels activated by pro-inflammatory treatment exhibited a neuroprotective effect against NMDA excitotocixity.
ATP released by electrical stimuli elicits calcium transients and gene expression in skeletal muscle.
Cannabinoids prevent the opposite regulation of astroglial connexin43 hemichannels and gap junction channels induced by pro-inflammatory treatments.
Dysfunctions of the diffusional membrane pathways mediated by hemichannels in inherited and acquired human diseases.
Gap junctions at the dendritic cell-T cell Interface are key elements for antigen-dependent T cells activation.
Metabolic inhibition increases the activity of connexin-32 hemichannels which are permeable to Ca2+
Modulation of brain hemichannels and gap junction channels by pro-inflammatory agents and their possible role in neuro-egeneration.
Astrocytes as connexin-dependent signaling cells for local blood flow regulation.
Currently used methods for identification and characterization of hemichannels
Functional role of gap junctions in cytokine-induced leukocyte adhesion and transmigration in vivo.
The connexin hemichannel composition determines the FGF-1-induced membrane permeability and free Ca2+i responses.
Biological mechanisms involved in the spread of traumatic brain damage.
Sepsis-Induced Channelopathy in Skeletal Muscles is Associated with Expression of Non-Selective Channels.

Article (73)

Extracellular gentamicin reduces the activity of connexin hemichannels and interferes with purinergic Ca(2+) signaling in HeLa cells.
Gap Junction Intercellular Communication Mediated by Connexin43 in Astrocytes Is Essential for Their Resistance to Oxidative Stress
Opening of pannexin- and connexin-based channels increases the excitability of nodose ganglion sensory neurons.
Pannexin 1 channels in skeletal muscles
Pannexin 1 channels: new actors in the regulation of catecholamine release from adrenal chromaffin cells.
Pannexin1 channels act downstream of P2X(7) receptors in ATP-induced murine T-cell death
ATP is Required and Advances Cytokine-Induced Gap Junction Formation in Microglia In Vitro
Boldine Prevents Renal Alterations in Diabetic Rats
Cerebral ischemic injury is enhanced in a model of oculodentodigital dysplasia
Connexin and pannexin hemichannels in brain glial cells: properties, pharmacology, and roles.
De novo expression of connexin hemichannels in denervated fast skeletal muscles leads to atrophy
Diffusion of nitric oxide across cell membranes of the vascular wall requires specific connexin-based channels
Disruption in connexin-based communication is associated with intracellular Ca²⁺ signal alterations in astrocytes from Niemann-Pick type C mice.
FasL-triggered death of Jurkat cells requires caspase 8-induced, ATP-dependent cross-talk between Fas and the purinergic receptor P2X(7)
HIV increases the release of dickkopf-1 protein from human astrocytes by a Cx43 hemichannel-dependent mechanism.
The ATP required for potentiation of skeletal muscle contraction is released via pannexin hemichannels
The ATP required for potentiation of skeletal muscle contraction is released via pannexin hemichannels.
Connexin 36 is Expressed in Beta and Connexins 26 and 32 in Acinar Cells at the End of the Secondary Transition of Mouse Pancreatic Development and Increase During Fetal and Perinatal Life
Connexin-based channels in astrocytes: How to study their properties
Critical role of the first transmembrane domain of Cx26 in regulating oligomerization and function
Glial hemichannels and their involvement in aging and neurodegenerative diseases
Glucose increases intracellular free Ca2+ in tanycytes via ATP released through connexin 43 hemichannels
Permeation of Calcium through Purified Connexin 26 Hemichannels
Regulation of Intercellular Calcium Signaling Through Calcium Interactions with Connexin-Based Channels
Release of gliotransmitters through astroglial connexin 43 hemichannels is necessary for fear memory consolidation in the basolateral amygdala
Amyloid ?-induced death in neurons involves glial and neuronal hemichannels
Amyloid beta-Induced Death in Neurons Involves Glial and Neuronal Hemichannels
ATP and glutamate released via astroglial connexin 43 hemichannels mediate neuronal death through activation of pannexin 1 hemichannels
Boldina disminuye la apoptosis miocárdica post isquemia reperfusión en la rata
Cation permeation through connexin 43 hemichannels is cooperative, competitive and saturable with parameters depending on the permeant species
De novo expression of connexin hemichannels in denervated fast skeletal muscles leads to atrophy.
Hemichannels in the Neurovascular Unit and White Matter Under Normal and Inflamed Conditions
FGF-1 induces ATP release from spinal astrocytes in culture and opens pannexin and connexin hemichannels
Hypoxia in High Glucose Followed by Reoxygenation in Normal Glucose Reduces the Viability of Cortical Astrocytes Through Increased Permeability of Connexin 43 Hemichannels
Inhibition of cytokine-induced connexin43 hemichannel activity in astrocytes is neuroprotective
ATP Released by Electrical Stimuli Elicits Calcium Transients and Gene Expression in Skeletal Muscle
Cannabinoids prevent the opposite regulation of astroglial connexin43 hemichannels and gap junction channels induced by pro-inflammatory treatments
Gap Junctions at the Dendritic Cell-T Cell Interface Are Key Elements for Antigen-Dependent T Cell Activation
Metabolic inhibition increases activity of connexin-32 hemichannels permeable to Ca2+ in transfected HeLa cells
Connexin hemichannel composition determines the FGF-1-induced membrane permeability and free [Ca2+](i) responses
Currently used methods for identification and characterization of hemichannels
Functional role of gap junctions in cytokine-induced leukocyte adhesion to endothelium in vivo
Cx43 hemichannels and gap junction channels in astrocytes are regulated oppositely by proinflammatory cytokines released from activated microglia
Functional gap junctions facilitate melanoma antigen transfer and cross-presentation between human dendritic cells
Inflammatory conditions induce gap junctional communication between rat Kupffer cells both in vivo and in vitro
Injury of skeletal muscle and specific cytokines induce the expression of gap junction channels in mouse dendritic cells
Opening of connexin 43 hemichannels is increased by lowering intracellular redox potential
Chlorpromazine reduces the intercellular communication via gap junctions in mammalian cells
S-nitrosylation and permeation through connexin 43 hemichannels in astrocytes: Induction by oxidant stress and reversal by reducing agents
Expression of connexins during differentiation and regeneration of skeletal muscle: functional relevance of connexin43
Role of connexin-based gap junction channels and hemichannels in ischemia-induced cell death in nervous tissue
Connexin43 is expressed in mouse fetal ovary
Functioning of Cx43 hemichannels demonstrated by single channel properties
Gating and regulation of connexin 43 (Cx43) hemichannels
Presence and importance of connexin43 during myogenesis
TNF-? plus IFN-? induce connexin43 expression and formation of gap junctions between human monocytes/macrophages that enhance physiological responses
Identification of second messengers that induce expression of functional gap junctions in microglia cultured from newborn rats
Metabolic inhibition induces opening of unapposed connexin 43 gap junction hemichannels and reduces gap junctional communication in cortical astrocytes in culture
Regulation of hepatic connexins in cholestasis: possible involvement of Kupffer cells and inflammatory mediators
Microglia at brain stab wounds express connexin 43 and in vitro form functional gap junctions after treatment with interferon-gamma and tumor necrosis factor-alpha
Gap junctions in cells of the immune system: structure, regulation and possible functional roles
Gap junctions in the chicken pineal gland
Regulation of astrocyte gap junctions by hypoxia-reoxygenation
Cells of the carotid body express connexin43 which is up-regulated by cAMP
Chapter 25: Gap Junctions in Inflammatory Responses: Connexins, Regulation and Possible Functional Roles
The ependymocytes of the bovine subcommissural organ are functionally coupled through gap junctions
Gap junctional communication coordinates vasopressin-induced glycogenolysis in rat hepatocytes
Identification of gap junctions in the oviduct and regulation of connexins during development and by sexual hormones
Phosphorylation of connexin43 and the regulation of neonatal rat cardiac myocyte gap junctions
Regulation of glycogen content in rat pineal gland by norepinephrine
C-erbB2/neu transfection induces gap junctional communication incompetence in glial cells
Regulated assembly of connexin33 and connexin43 into rat Sertoli cell gap junctions

Generic (13)

HIV infection of astrocytes increases release of Dickkopf-1 protein by a gap junction and hemichannel dependent mechanism
CONNEXIN 43 HEMICHANNELS MEDIATE THE CA(2+) INFLUX INDUCED BY EXTRACELLULAR ALKALINIZATION
Dysfunctions of the Diffusional Membrane Pathways Mediated Hemichannels in Inherited and Acquired Human Diseases
METABOLIC INHIBITION INCREASES ACTIVITY OF CONNEXIN-32 HEMICHANNELS PERMEABLE TO CA(2+) IN TRANSFECTED HELA CELLS
HISTAMINE REDUCES GAP JUNCTIONAL COMMUNICATION OF HUMAN TONSIL HIGH ENDOTHELIAL CELLS IN CULTURE
THE FORMATION OF SKELETAL MUSCLE MYOTUBES REQUIRES FUNCTIONAL MEMBRANE RECEPTORS ACTIVATED BY EXTRACELLULAR ATP
UNIONES EN HENDIDURA Y SU PAPEL FUNCIONAL EN EL TRACTO REPRODUCTOR FEMENINO
GAP JUNCTION HEMICHANNELS IN ASTROCYTES OF THE CNS
ACTIVATION OF HUMAN POLYMORPHONUCLEAR CELLS INDUCES FORMATION OF FUNCTIONAL GAP JUNCTIONS AND EXPRESSION OF CONNEXINS
Specific ablation of connexin 43 delays skeletal muscle regeneration in vivo
EXPRESSION OF CONNEXIN43 IN MOUSE LEYDIG, SERTOLI, AND GERMINAL CELLS AT DIFFERENT STAGES OF POSTNATAL DEVELOPMENT
REGULATION OF GAP-JUNCTIONS BY PROTEIN PHOSPHORYLATION

Review (12)

Hunting for connexin hemichannels
Possible Involvement of TLRs and Hemichannels in Stress-Induced CNS Dysfunction via Mastocytes, and Glia Activation
Role of Gap Junctions and Hemichannels in Parasitic Infections
Biological mechanisms involved in the spread of traumatic brain damage
Connexin and pannexin hemichannels in inflammatory responses of glia and neurons
Connexin- and Pannexin-Based Channels in Normal Skeletal Muscles and Their Possible Role in Muscle Atrophy
Glial connexin expression and function in the context of Alzheimer's disease
Modulation of gap junction channels and hemichannels by growth factors
Cell membrane permeabilization via connexin hemichannels in living and dying cells
Possible involvement of different connexin43 domains in plasma membrane permeabilization induced by ischemia-reperfusion
Connexin-based gap junction hemichannels: Gating mechanisms
New roles for astrocytes: Gap junction hemichannels have something to communicate

Editorial Material (2)

We've had important advances in the connexin/pannexin field, yet there is still much to do
Astrocytes as connexin-dependent signaling cells for local blood flow regulation

Conference Paper (1)

Brain Research Reviews: Preface
129
Juan Saez

Professor

Physiology

Pontificia Universidad Católica de Chile

Santiago, Chile

27
Juan Orellana

Assistant Professor

Departamento de Neurología

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE

Santiago, Chile

17
Mauricio Retamal

Profesor Asociado

Centro de Fisiología Celular e Integrativa

Universidad del Desarrollo

Santiago, Chile

15
Juan Sáez

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE

Santiago, Chile

5
Xavier Figueroa

Associate Professor

Departamento de Fisiologia

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE

Santiago, Chile

4
Vania Figueroa

Profesor Asistente

Instituto de Ciencias de la Salud

Universidad de O’Higgins

Rancagua, Chile

4
Jose Luis Vega

Profesor Asociado

Biomedico

Universidad de Antofagasta

Antofagasta, Chile

3
Rommy Von Bernhardi

Full Professor

Neurology

DEPT. NEUROLOGY, FAC. MEDICINE PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE

Santiago, Chile

3
Claudia Saez

Asistant Professor

Hematology-Oncology

Pontificia Universidad Católica de Chile

Santiago, Chile

3
Luis Cea

Profesor programa teaching

Instituto de Ciencias Biomédicas

Universidad Autónoma de Chile

Santiago, Chile

2
Carlos Puebla

Profesor Asistente

Universidad Autónoma de Chile

Santiago, Chile

2
HELMUTH SANCHEZ

INVESTIGADOR JOVEN

Laboratorio de Conexinas y Panexinas

CENTRO INTERDISCIPLINARIO DE NEUROCIENCIAS DE VALPARAISO

VALPARAISO, Chile

2
Agustin Martinez

Profesor Titular

Instituto de Neurociencia

UNIVERSIDAD DE VALPARAÍSO, CENTRO INTERDISCIPLINARIO DE NEUROCIENCIAS DE VALPARAÍSO

Valparaíso, Chile

2
Loreto Véliz

Profesor Asistente Adjunto

Fisiología

Pontificia Universidad Católica de Chile

Santiago, Chile

2
Mariana Casas

Assistant Professor

FACULTAD DE MEDICINA, UNIVERSIDAD DE CHILE

Santiago, Chile

2
Alexis Kalergis

Full Professor

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE. FACULTAD DE CIENCIAS BIOLÓGICAS

Santiago, Chile

1
Marlene Arismendi

INVESTIGADOR JEFE

INVESTIGACION Y DESARROLLO

NANOTEC S.A

SANTIAGO, Chile

1
CARLOS LAGOS

Profesor Asistente

FACULTAD DE MEDICINA Y CIENCIA

UNIVERSIDAD SAN SEBASTIÁN

SANTIAGO, Chile

1
Tomas Perez-Acle

Full Professor

Computational Biology Lab

Fundación Ciencia para la Vida

Santiago, Chile

1
Mercedes Lopez

Profesora Asociada

Instituto de Ciencias Biomédicas

Universidad de chile

Santiago, Chile

1
Jorge Gonzalez

Profesor Titular

Departamento de Tecnología Médica

UNIVERSIDAD DE ANTOFAGASTA

Antofagasta, Chile

1
Felipe Simon

Profesor Titular

Department of Biological Sciences

UNIVERSIDAD ANDRES BELLO FAC DE CS BIOLOGICAS, DPTO DE CS BIOLOGICAS

Santiago, Chile

1
Jimmy Stehberg

Associate Professor

Biological Sciences

UNIVERSIDAD ANDRÉS BELLO

SANTIAGO, Chile

1
Maria Garcia

Profesor Titular

Biología Celular

UNIVERSIDAD DE CONCEPCIÓN

Concepción, Chile

1
Isaac Garcia

Profesor Adjunto

Instituto de Neurociencias

Universidad de Valparaiso

Valparaiso, Chile

1
Alejandra Vielma

Post-doctorado

Ciencias Biológicas, Ciencias Fisiológicas

Pontificia Universidad Católica de Chile

Santiago, Chile

1
Maria Ocaranza

Asociate Professor

Cardiovascular Diseases Division

Pontificia Universidad Catolica de Chile

Santiago, Chile

1
Sonja Buvinic

Assistant Professor

Institute for Research in Dental Sciences

Universidad de Chile

Santiago, Chile

1
Mario Rosemblatt

Executive Director

-

Fundacion Ciencia & Vida

Santiago, Chile

1
Roberto Elizondo

Investigador Post-Doctoral

Universidad de los Andes

Santiago, Chile

1
Marcela Hermoso

Full Professor

School of Medicine

UNIVERSIDAD DE CHILE

Santiago, Chile

1
Francisco Nualart

Full Professor and Scientific Director

Cellular Biology and Center for Advanced Microscopy CMA BIO-BIO

UNIVERSIDAD DE CONCEPCION

Concepcion, Chile

1
Jaime De Calisto

Director Docente

Escuela de Biotecnología

Universidad Mayor

Santiago, Chile

1
Enrique Jaimovich

Full Professor

ICBM

FACULTAD DE MEDICINA, UNIVERSIDAD DE CHILE

Santiago, Chile

1
Hernan Gonzalez Diaz

Associate Professor

Cirugía Oncológica

Pontificia Universidad Católica de Chile

Santiago, Chile

1
Margarita Pizarro

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE

Santiago, Chile